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| Status |
Public on Jan 09, 2023 |
| Title |
Conserved and plant-specific GCN5-containing complexes cooperate to regulate gene transcription and plant development [ChIP-Seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Although the conserved histone acetyltransferase HAG1/GCN5-containing complex SAGA (Spt-Ada-Gcn5 acetyltransferase) has been extensively studied in plants, whether HAG1 forms a plant-specific complex is unknown. Here, we identified a plant-specific GCN5-containing complex, PAGA (plant-ADA2A-GCN5-acetyltransferase), in Arabidopsis thaliana; the complex consists of two conserved subunits (HAG1/GCN5 and ADA2A) and four plant-specific subunits (SPC, ING1, SDRL, and EAF6). In the PAGA complex, SPC functions as a scaffold protein that is responsible for integrating the other subunits. SPC also enhances the binding of ING1 to histone H3 with methylated lysine 4 and promotes the histone acetylation activity of HAG1. Chromatin immunoprecipitation followed by sequencing indicated that PAGA not only shares target genes with SAGA but also has its specific target genes. While PAGA and SAGA promote transcription by mediating moderate and high levels of histone acetylation, respectively, at different sets of genes, PAGA can also function antagonistically against SAGA to prevent excessive transcription of PAGA- and SAGA-shared target genes. Unlike SAGA, which regulates multiple biological processes, PAGA is mainly involved in plant height and branch growth by regulating the transcription of genes involved in hormone biosynthesis and response. These results indicate that the HAG1-containing SAGA and PAGA complexes are coordinated to regulate gene transcription and development.
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| Overall design |
Examination of the enrichment of ADA2A, ADA2B, HAG1, and ING1 on chromatin; Examination of H3K9Ac levels in wild type and two mutants
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| Contributor(s) |
He X |
| Citation(s) |
36879016 |
| Submission date |
Mar 30, 2022 |
| Last update date |
Apr 10, 2023 |
| Contact name |
xinjian He |
| E-mail(s) |
hexinjian@nibs.ac.cn
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| Organization name |
National Institute of Biological Sciences
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| Lab |
hexinjian
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| Street address |
No. 7, zhongguancun life science park road, changping district
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| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
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| Platforms (1) |
| GPL26208 |
Illumina NovaSeq 6000 (Arabidopsis thaliana) |
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| Samples (18)
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| GSM5983942 |
ADA2B-GFP, input |
| GSM5983943 |
ADA2B-GFP, IP, rep1 |
| GSM5983944 |
ADA2B-GFP, IP, rep2 |
| GSM5983945 |
GCN5-GFP, input |
| GSM5983946 |
GCN5-GFP, IP, rep1 |
| GSM5983947 |
GCN5-GFP, IP, rep2 |
| GSM5983948 |
ING1-GFP, input |
| GSM5983949 |
ING1-GFP, IP, rep1 |
| GSM5983950 |
ING1-GFP, IP, rep2 |
| GSM5983951 |
ada2a, H3K9Ac, rep1 |
| GSM5983952 |
ada2a, H3K9Ac, rep2 |
| GSM5983953 |
Col-0, H3K9Ac, rep1 |
| GSM5983954 |
Col-0, H3K9Ac, rep2 |
| GSM5983955 |
spcing1, H3K9Ac, rep1 |
| GSM5983956 |
spcing1, H3K9Ac, rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE199761 |
Conserved and plant-specific GCN5-containing complexes cooperate to regulate gene transcription and plant development |
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| Relations |
| BioProject |
PRJNA821459 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199757_RAW.tar |
804.6 Mb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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