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| Status |
Public on Nov 03, 2022 |
| Title |
Patterns of heterochromatin distribution alterations linked to transcriptional changes at Plasmodium falciparum clonally variant gene loci [gene expression] |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by array
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| Summary |
The survival of malaria parasites in the changing human blood environment largely depends on their ability to alter gene expression by epigenetic mechanisms. The active state of Plasmodium falciparum clonally variant genes is associated with euchromatin characterized by the histone mark H3K9ac, whereas the silenced state is characterized by H3K9me3-based heterochromatin. However, the localization of the euchromatin-heterochromatin transitions associated with expression switches in different clonally variant genes has not been characterized. Here we compared the distribution of heterochromatin between subclones of the same genetic background with different patterns of expressed and silenced clonally variant genes to identify at a genome-wide level the patterns associated with the different transcriptional states. We found that de novo heterochromatin formation or complete disruption of a heterochromatin domain are relatively rare events, and in the majority of loci expression switches can be explained by expansion or retraction of heterochromatin. We describe different modalities of heterochromatin changes linked to transcriptional differences, revealing a complex scenario. Despite this complexity, heterochromatin distribution patterns generally enable prediction of the transcriptional state of clonally variant genes. Some subclones expressed and had in an active chromatin state several var genes simultaneously. We also found that heterochromatin levels in the putative regulatory region of the gdv1-as non-coding RNA, previously involved in sexual commitment, varied between parasite lines with different sexual conversion rates.
Gene expression data for subclones A7, E5 and B11 (3D7-B stock).
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| Overall design |
Time-course analysis for 3D7-B subclones: A7, E5 and B11. Samples for transcriptomic analysis of A7, E5 and B11 lines were obtain from tightly synchronized cultures (0-5 h window) achieved by Percoll purification of erythrocytes infected with mature stages followed by 5% sorbitol lysis 5 h later. RNA was extracted from samples collected at 10-15, 20-25, 30-35, 37-42, 40-45 and 43-48 h post-invasion (hpi) using the Trizol method. The reference pool consisted of a mixture of equal amounts of cDNA from rings, trophozoites and schizonts from 3D7-A and 3D7-B stocks. Samples were hybridized oncustom Agilent microarrays (AMADID no. 085763) modified from design AMADID no. 037237.
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| Contributor(s) |
Rovira-Graells N, Casas-Vila N, Michel-Todó L, Cortés A |
| Citation(s) |
36515553 |
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| Submission date |
Jul 13, 2022 |
| Last update date |
Feb 02, 2023 |
| Contact name |
Lucas Michel-Todó |
| E-mail(s) |
lucas.michel@isglobal.org
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| Organization name |
ISGlobal
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| Department |
Malaria Epigenetics
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| Street address |
Roselló, 149
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| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
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| Platforms (1) |
| GPL26985 |
Agilent-085763 Pfalciparum15KArray_Sept18 |
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| Samples (18)
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| GSM6337846 |
A7, timepoint 37-42 hpi |
| GSM6337847 |
A7, timepoint 40-45 hpi |
| GSM6337848 |
A7, timepoint 43-48 hpi |
| GSM6337849 |
E5, timepoint 10-15 hpi |
| GSM6337850 |
E5, timepoint 20-25 hpi |
| GSM6337851 |
E5, timepoint 30-35 hpi |
| GSM6337852 |
E5, timepoint 37-42 hpi |
| GSM6337853 |
E5, timepoint 40-45 hpi |
| GSM6337854 |
E5, timepoint 43-48 hpi |
| GSM6337855 |
B11, timepoint 10-15 hpi |
| GSM6337856 |
B11, timepoint 20-25 hpi |
| GSM6337857 |
B11, timepoint 30-35 hpi |
| GSM6337858 |
B11, timepoint 37-42 hpi |
| GSM6337859 |
B11, timepoint 40-45 hpi |
| GSM6337860 |
B11, timepoint 43-48 hpi |
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| This SubSeries is part of SuperSeries: |
| GSE208561 |
Patterns of heterochromatin distribution alterations linked to transcriptional changes at Plasmodium falciparum clonally variant gene loci |
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| Relations |
| BioProject |
PRJNA858441 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE208131_RAW.tar |
28.9 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE208131_processed_data_with_genename.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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