GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE221679 Query DataSets for GSE221679
Status Public on Jun 09, 2023
Title The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation.
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary MOM1 is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we found that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with artificial zinc finger to unmethylated FWA promoter led to establishment of DNA methylation and FWA silencing. This process was blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MORC6. We found that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes was impaired by mutation of MOM1 or mutation of genes encoding the MOM1 interacting PIAL1/2 proteins. In addition to RdDM sites, we identified a group of MOM1 peaks at active chromatin near genes, again colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation.
Overall design This dataset includes ChIP-seq, RNA-seq, WGBS, ATAC-seq and BS-PCR to understand the role of MOM1 complex, especially the connection of MOM1 complex to the RdDM machinery and the MORC proteins.
Contributor(s) Li Z, Wang M, Zhenhui Z
Citation(s) 37438334
Submission date Dec 23, 2022
Last update date Sep 08, 2023
Contact name Zhenhui Zhong
Organization name University of California, Los Angeles
Department Department of Molecular, Cell and Developmental Biology
Lab Jacobsen Lab
Street address 610 Charles E Young Dr East
City Los Angeles
ZIP/Postal code 90095
Country USA
Platforms (2)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
GPL26208 Illumina NovaSeq 6000 (Arabidopsis thaliana)
Samples (125)
GSM6892915 ATAC seq data of wild type (Col-0) replicate 1
GSM6892916 ATAC seq data of wild type (Col-0) replicate 2
GSM6892917 ATAC seq data of mom1-3 mutant replicate 1
BioProject PRJNA915364

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE221679_RAW.tar 6.1 Gb (http)(custom) TAR (of BW, NARROWPEAK, RESULTS, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap