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Series GSE24934 Query DataSets for GSE24934
Status Public on Jan 12, 2011
Title The knockdown of the maternal estrogen receptor-β2 mRNA (ers2a) affects embryo transcript contents and larval development in zebrafish
Organism Danio rerio
Experiment type Expression profiling by array
Summary In zebrafish, vitellogenic oocytes can incorporate significant amounts of 17β-estradiol released from nearby granulosa cells according to a first-order kinetics, since the steroid low polarity ensures high permeability and affinity for yolk lipids. Estrogen bioactivity is likely, because the maternal mRNA for the estrogen receptor-β2 (ers2a) is highly expressed in ovulated oocytes. This transcript is available for translation in the embryo until its sharp decline from 4 to 8 hours post-fertilization (hpf), being replaced by low levels of zygotic ers2a mRNA from 24 hpf to hatching at 48 hpf, as determined by qRT-PCR. Estrogen receptors-α and -β1 are only expressed zygotically at low levels from 24 hpf onwards. To test the functional role of maternal ers2a mRNA, 1- or 2-cell embryos were injected with 10.3 ng each of morpholino to knockdown translation (MO2-ers2a) of both maternal and zygotic ers2a transcripts, missplicing morpholino (MO3-ers2a) to block post-transcriptionally the zygotic transcript alone, and a nonspecific morpholino (MO-control) as a control. Treatment with MO2-ers2a caused severe malformations in 63% of 1-5 dpf larvae, as compared to 10-11% in those treated with MO3-ers2a and MO2-control. Defects included body growth delay and curved shape, abnormal brain and splanchnocranium development, enlarged and hemorrhagic pericardial cavity, uninflated swim bladder and rudimentary caudal fin with aberrant circular motion. Affected larvae could survive for only 12-14 days. Co-injection of an anti-p53 MO failed to rescue the MO2-ers2a-phenotypes, eliminating the possibility of off-target effects. Pangenomic microarray analysis revealed that 240 and 219 significantly expressed transcripts were up- and down-regulated, respectively, by maternal Ers2a protein deficiency in 8-hpf MO2-ers2a-embryos. Also at 48 hpf, 162 and 120 presumably zygotic transcripts were up- and down-regulated, respectively, but only 18 were in common with each of the 8-hpf sets. Whole-mount in situ hybridization revealed an intensified expression of the genes six3.1 and emx1 in MO2-ers2a-embryos at 24-48 hpf, as compared to controls. These findings suggest the involvement of maternal ers2a mRNA in the epigenetic programming of zebrafish development.
Overall design MO2-ers2a morphants were compared with MO-control at 8 hpf and 48 hpf. MO2-ers2a is a morpholinos selected to knockdown translation of ers2a mRNA
Contributor(s) Celeghin A, Francesca B, Pikulkaew S, Rabbane M, Colombo L, Dalla Valle L
Citation(s) 21199655
Submission date Oct 26, 2010
Last update date Dec 06, 2012
Contact name Andrea Celeghin
Phone +39 049 8276186
Organization name University of Padua
Department Biology
Lab Comparative Endocrinology
Street address Via Ugo Bassi 58 B
City Padua
State/province Veneto
ZIP/Postal code 35131
Country Italy
Platforms (1)
GPL6457 Agilent-019161 D. rerio (Zebrafish) Oligo Microarray (V2) G2519F (Feature Number version)
Samples (2)
GSM612962 MO2-ers2a 8 hpf - MO-control 8 hpf
GSM612963 MO2-ers2a 48 hpf - MO-control 48 hpf
BioProject PRJNA131927

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Supplementary file Size Download File type/resource
GSE24934_RAW.tar 5.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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