|
| Status |
Public on Mar 30, 2011 |
| Title |
Expression data from an in vitro growth of Methanobrevibacter smithii PS |
| Platform organisms |
Bacteroides thetaiotaomicron; Phocaeicola vulgatus; Parabacteroides distasonis; Methanobrevibacter smithii; Lachnospira eligens; Agathobacter rectalis |
| Sample organism |
Methanobrevibacter smithii ATCC 35061 |
| Experiment type |
Expression profiling by array
|
| Summary |
RNA-sequencing is a powerful tool for exploring expression profiles of microbial species that does not require the production of a custom genechip.
We used microarrays to compare our quantitative RNA-sequencing method to the standard expression quantification methods
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| |
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| Overall design |
Methanobrevibacter smithii PS cultures were grown to stationary phase under 80% hydrogen, 20% CO2, with varying amounts of formate. RNA was extracted and purified, then divided into two aliquots per sample. One aliquot was prepared for hybridization on Affymetrix gene chips, the other was prepared for microbial RNA-sequencing.
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| |
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| Contributor(s) |
Rey F, Hansen E |
| Citation(s) |
21317366 |
| |
| Submission date |
Nov 22, 2010 |
| Last update date |
Jan 30, 2015 |
| Contact name |
Federico E Rey |
| E-mail(s) |
reyf@wustl.edu
|
| Phone |
314-963-0284
|
| Organization name |
Washington University
|
| Department |
Pathology
|
| Lab |
Gordon
|
| Street address |
4444 Forest Park Blvd
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7006 |
[BacEubMsa520350F] Affymetrix Human gut microbiota community GeneChip |
|
| Samples (4)
|
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| This SubSeries is part of SuperSeries: |
| GSE25537 |
Methanobrevibacter smithii expression profiling |
|
| Relations |
| BioProject |
PRJNA142435 |
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