|
| Status |
Public on Mar 30, 2011 |
| Title |
MsmPS48h_HL2 |
| Sample type |
RNA |
| |
|
| Source name |
Methanobrevibacter smithii PS in vitro culture
|
| Organism |
Methanobrevibacter smithii ATCC 35061 |
| Characteristics |
agent: 2.8mM formate
time: 48 h post innoculation
|
| Treatment protocol |
Aliquots were combined with an equal volume of RNA-Protect (QIAGEN), incubated for 5 min at room temperature, and then centrifuged to pellet.
|
| Growth protocol |
48 hour cultures were grown in modified MBC media under 30psi of 80% hydrogen and 20% CO2, replenished every 6 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
acid-phenol chloroform; mega clear kit
|
| Label |
biotin
|
| Label protocol |
Enzo Biochemicals Bioarray terminal labeling kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip BacEubMsa custom Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from cultures in log phase growth with 2.8mM formate
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Nov 22, 2010 |
| Last update date |
Mar 30, 2011 |
| Contact name |
Federico E Rey |
| E-mail(s) |
reyf@wustl.edu
|
| Phone |
314-963-0284
|
| Organization name |
Washington University
|
| Department |
Pathology
|
| Lab |
Gordon
|
| Street address |
4444 Forest Park Blvd
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL7006 |
| Series (2) |
| GSE25535 |
Expression data from an in vitro growth of Methanobrevibacter smithii PS |
| GSE25537 |
Methanobrevibacter smithii expression profiling |
|
