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| Status |
Public on May 25, 2011 |
| Title |
ChIP-chip of BMP2 or control treated human pulmonary artery endothelial cells with anti-beta-catenin or anti-ppar-gamma antibodies on promoter regions |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Reduced bone morphogenetic protein receptor (BMPR)2 expression in patients with pulmonary arterial (PA) hypertension (PAH), can impair PA endothelial cell (EC) function. We now characterize, in human PAECs, a novel BMPR2-mediated transcriptionally active complex between peroxisome proliferator-activated receptor (PPAR) gamma and beta-catenin (BC), and show that disruption of this complex impairs BMP mediated HPAEC survival.
Using whole genome wide ChIP-Chip promoter analysis we delineate PPARG-BC dependent transcription of target genes that include apelin.
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| Overall design |
Comparison of ppar-gamma and beta-catenin occupancy on promoter regions from human pulmonary artery endothelial cells after either treatment with BMP2 (10ng/ml) or control. A total of 8 samples were created using NimbleGen human HG18 promoter arrays.
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| Contributor(s) |
Alastalo T, Li M, Rabinovitch M |
| Citation(s) |
21821917 |
| |
| Submission date |
May 23, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Molong Li |
| E-mail(s) |
molong.li@jhmi.edu
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| Organization name |
Johns Hopkins University
|
| Department |
School of Medicine
|
| Street address |
733 N. Broadway
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (2) |
| GPL6325 |
NimbleGen Homo sapiens HG18 promoter 1 of 2 |
| GPL6326 |
NimbleGen Homo sapiens HG18 promoter 2 of 2 |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA141651 |
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