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Status |
Public on May 25, 2011 |
Title |
ChIP-chip of BMP2 or control treated human pulmonary artery endothelial cells with anti-beta-catenin or anti-ppar-gamma antibodies on promoter regions |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
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Summary |
Reduced bone morphogenetic protein receptor (BMPR)2 expression in patients with pulmonary arterial (PA) hypertension (PAH), can impair PA endothelial cell (EC) function. We now characterize, in human PAECs, a novel BMPR2-mediated transcriptionally active complex between peroxisome proliferator-activated receptor (PPAR) gamma and beta-catenin (BC), and show that disruption of this complex impairs BMP mediated HPAEC survival. Using whole genome wide ChIP-Chip promoter analysis we delineate PPARG-BC dependent transcription of target genes that include apelin.
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Overall design |
Comparison of ppar-gamma and beta-catenin occupancy on promoter regions from human pulmonary artery endothelial cells after either treatment with BMP2 (10ng/ml) or control. A total of 8 samples were created using NimbleGen human HG18 promoter arrays.
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Contributor(s) |
Alastalo T, Li M, Rabinovitch M |
Citation(s) |
21821917 |
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Submission date |
May 23, 2011 |
Last update date |
Mar 23, 2012 |
Contact name |
Molong Li |
E-mail(s) |
molong.li@jhmi.edu
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Organization name |
Johns Hopkins University
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Department |
School of Medicine
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Street address |
733 N. Broadway
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City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21205 |
Country |
USA |
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Platforms (2) |
GPL6325 |
NimbleGen Homo sapiens HG18 promoter 1 of 2 |
GPL6326 |
NimbleGen Homo sapiens HG18 promoter 2 of 2 |
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Samples (8)
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Relations |
BioProject |
PRJNA141651 |