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Series GSE29489 Query DataSets for GSE29489
Status Public on May 25, 2011
Title ChIP-chip of BMP2 or control treated human pulmonary artery endothelial cells with anti-beta-catenin or anti-ppar-gamma antibodies on promoter regions
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Reduced bone morphogenetic protein receptor (BMPR)2 expression in patients with pulmonary arterial (PA) hypertension (PAH), can impair PA endothelial cell (EC) function. We now characterize, in human PAECs, a novel BMPR2-mediated transcriptionally active complex between peroxisome proliferator-activated receptor (PPAR) gamma and beta-catenin (BC), and show that disruption of this complex impairs BMP mediated HPAEC survival.
Using whole genome wide ChIP-Chip promoter analysis we delineate PPARG-BC dependent transcription of target genes that include apelin.
Overall design Comparison of ppar-gamma and beta-catenin occupancy on promoter regions from human pulmonary artery endothelial cells after either treatment with BMP2 (10ng/ml) or control. A total of 8 samples were created using NimbleGen human HG18 promoter arrays.
Contributor(s) Alastalo T, Li M, Rabinovitch M
Citation(s) 21821917
Submission date May 23, 2011
Last update date Mar 23, 2012
Contact name Molong Li
Organization name Johns Hopkins University
Department School of Medicine
Street address 733 N. Broadway
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (2)
GPL6325 NimbleGen Homo sapiens HG18 promoter 1 of 2
GPL6326 NimbleGen Homo sapiens HG18 promoter 2 of 2
Samples (8)
GSM729614 BCTNN_BMP2_1of2
GSM729615 BCTNN_CON_1of2
GSM729616 PPARG_BMP2_1of2
BioProject PRJNA141651

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29489_RAW.tar 202.4 Mb (http)(custom) TAR (of GFF, PAIR)
Processed data included within Sample table
Processed data provided as supplementary file

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