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| Status |
Public on Dec 31, 2011 |
| Title |
AhNAS2 suppression in Arabidopsis halleri roots |
| Platform organism |
Arabidopsis thaliana |
| Sample organism |
Arabidopsis halleri |
| Experiment type |
Expression profiling by array
|
| Summary |
The model Zn hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to non-accumulators among a core of alterations in metal homeostasis gene expression. Suppression of AhNAS2 expression by RNAi resulted in strongly reduced root NA accumulation and a concomitant decrease in root-to-shoot-translocation of Zn but not of other micronutrients. Few secondary effects on the root transcriptome were detected by microarray analysis.
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| Overall design |
Vegetatively propagated clones of A. halleri wildtype plants and a line with strong suppression of AhNAS2 were grown hydroponically for weeks in two different media (control Hoagland and 10 µM Zn2+ added). Roots were harvested and analyzed. Three plants were pooled for one sample. Three independent experiments were performed.
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| Contributor(s) |
Deinlein U, Clemens S |
| Citation(s) |
22374395 |
| Submission date |
Aug 31, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Stephan Clemens |
| E-mail(s) |
stephan.clemens@uni-bayreuth.de
|
| Organization name |
University of Bayreuth
|
| Department |
Plant Physiology
|
| Street address |
Universitaetsstrasse 30
|
| City |
Bayreuth |
| ZIP/Postal code |
95440 |
| Country |
Germany |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA145285 |
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