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Series GSE31778 Query DataSets for GSE31778
Status Public on Dec 31, 2011
Title AhNAS2 suppression in Arabidopsis halleri roots
Platform organism Arabidopsis thaliana
Sample organism Arabidopsis halleri
Experiment type Expression profiling by array
Summary The model Zn hyperaccumulators Arabidopsis halleri and Noccaea caerulescens share elevated nicotianamine synthase (NAS) expression relative to non-accumulators among a core of alterations in metal homeostasis gene expression. Suppression of AhNAS2 expression by RNAi resulted in strongly reduced root NA accumulation and a concomitant decrease in root-to-shoot-translocation of Zn but not of other micronutrients. Few secondary effects on the root transcriptome were detected by microarray analysis.
 
Overall design Vegetatively propagated clones of A. halleri wildtype plants and a line with strong suppression of AhNAS2 were grown hydroponically for weeks in two different media (control Hoagland and 10 ┬ÁM Zn2+ added). Roots were harvested and analyzed. Three plants were pooled for one sample. Three independent experiments were performed.
 
Contributor(s) Deinlein U, Clemens S
Citation(s) 22374395
Submission date Aug 31, 2011
Last update date Aug 15, 2018
Contact name Stephan Clemens
E-mail(s) stephan.clemens@uni-bayreuth.de
Organization name University of Bayreuth
Department Plant Physiology
Street address Universitaetsstrasse 30
City Bayreuth
ZIP/Postal code 95440
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (12)
GSM788390 WT-Root-Control-1
GSM788391 WT-Root-Control-2
GSM788392 WT-Root-Control-3
Relations
Affiliated with GSE69995
BioProject PRJNA145285

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31778_RAW.tar 22.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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