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| Status |
Public on Nov 01, 2005 |
| Title |
Differential gene expression of ydgG mutant |
| Platform organism |
Escherichia coli K-12 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
The E. coli Genechip antisense genome array was used to study the differential gene expression profile of E. coli K12 ydgG mutant compared to its isogenic wild type in a mature biofilm.
Keywords: E. coli K12 antisense chip
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| Overall design |
Overnight cultures were diluted 100X into 1 L shake flasks containing 250 mL of LB + 0.2 % and 10 g of glass wool. The cells were shaken at 250 rpm and 37 C for 24 hours (final OD600=5 for the suspended cells), to form a biofilm on the glass wool, and RNA was isolated from the biofilm and Affymetrix protocol was used for preparing fragmented and labelled cDNA that was hybrizide to the chips.
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| Contributor(s) |
Herzberg M, Kaye IK, Peti W, Wood TK |
| Citation(s) |
16385049 |
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| Submission date |
Oct 26, 2005 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Thomas K Wood |
| E-mail(s) |
twood@engr.uconn.edu
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| Organization name |
University of Connecticut
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| Department |
Chemical Engineering
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| Street address |
191 Auditorium Rd
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| City |
Storrs |
| State/province |
CT |
| ZIP/Postal code |
06269 |
| Country |
USA |
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| Platforms (1) |
| GPL199 |
[Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA93559 |
