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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 24, 2006 |
| Title |
MicroRNA-134 Modulates Mouse Embryonic Stem Cell Differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
Expression profile of miRNAs during retinoic acid (RA) induced differentiation of mose embryonic stem cells
Expression profile of mRNAs during retinoic acid (RA) amd miR-134 induced differentiation of mouse embryonic stem cells.
Keywords: affymetrix custom array and illumina MouseRef-8
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| Overall design |
RNA was extracted from mouse embryonic stem cells (mESC ) treated with retinoic acid (RA) over specified time periods (Day 0, 1, 4) and profiled using acustom microarray designed by Affymetrix. Two biological repeats for every time point was measured.
RNA was extracted from mESCs (Day 0, 2, 4 and 6) as well as from mESCs transfected with miR-134 precursor (134MM; 100nM) or scrambled oligomer or Lipofectamine only (MT) at Day3 post transfection. Two biological repeats for every time point or transfection. These RNA was then profiles using illumina beadarray MouseRef-8
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| Contributor(s) |
Tay Y, Tam W, Ang Y, Yeap L, Koo S, Sun Y, Koo S, Lim SK, Lou Y, Zhang J, Mitchell WP, Rigoutsos I, Lim B |
| Citation(s) |
17916804 |
| Submission date |
Mar 22, 2006 |
| Last update date |
Feb 11, 2020 |
| Contact name |
Bing Lim |
| E-mail(s) |
limb1@gis.a-star.edu.sg
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| Phone |
+65 64788156
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| Fax |
+65 64789005
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| URL |
http://www.gis.a-star.edu.sg
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| Organization name |
Genome Institute of Singapore
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| Department |
Stem Cell and Developmental Biology
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| Lab |
Stem Cell and Developmental Biology
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| Street address |
60 Biopolis street, #02-01 Genome
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| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
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| Platforms (2) |
| GPL3576 |
Affymetrix custom microarray to profile mirna |
| GPL4865 |
Sentrix MouseRef-8 Expression BeadChip (Target ID) |
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| Samples (30)
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| GSM101268 |
minus RA treatment replicate 1 (affy) |
| GSM101269 |
minus RA treatment replicate 2 (affy) |
| GSM101270 |
plus 100nM RA treatment, 24h, replicate 1 (affy) |
| GSM101271 |
plus 100nM RA treatment, 24h, replicate 2 (affy) |
| GSM101272 |
plus 100nM RA treatment, 96h, replicate 1 (affy) |
| GSM101273 |
plus 100nM RA treatment, 96h, replicate 2 (affy) |
| GSM101285 |
minus RA treatment replicate 1 |
| GSM101286 |
minus RA treatment replicate 2 |
| GSM101287 |
plus 100nM RA treatment, 48h, replicate 1 |
| GSM101288 |
plus 100nM RA treatment, 48h, replicate 2 |
| GSM101289 |
plus 100nM RA treatment, 96h, replicate 1 |
| GSM101290 |
plus 100nM RA treatment, 96h, replicate 2 |
| GSM101291 |
plus 100nM RA treatment, 144h, replicate 1 |
| GSM101292 |
plus 100nM RA treatment, 144h, replicate 2 |
| GSM101293 |
mock transfection; Lipofectamine only replicate 1 |
| GSM101294 |
mock transfection; Lipofectamine only replicate 2 |
| GSM101295 |
100nM Scrambled oligo transfection replicate 1 |
| GSM101296 |
100nM Scrambled oligo transfection replicate 2 |
| GSM101297 |
100nM miR134-mimetic transfection replicate 1 |
| GSM101298 |
100nM miR134-mimetic transfection replicate 2 |
| GSM104061 |
100nM Let7i-Pre-miR transfection replicate 1 |
| GSM104062 |
100nM Let7i-Pre-miR transfection replicate 2 |
| GSM104063 |
100nM Scrambled oligo transfection plus 100nM RA treatment, 48hr, replicate 1 |
| GSM104064 |
100nM Scrambled oligo transfection plus 100nM RA treatment, 48hr, replicate 2 |
| GSM104065 |
100nM Scrambled oligo transfection plus 100nM RA treatment, 72hr, replicate 1 |
| GSM104066 |
100nM Scrambled oligo transfection plus 100nM RA treatment, 72hr, replicate 2 |
| GSM104067 |
100nM Anti-miR-134 transfection plus 100nM RA treatment, 48hr, replicate 1 |
| GSM104068 |
100nM Anti-miR-134 transfection plus 100nM RA treatment, 48hr, replicate 2 |
| GSM104069 |
100nM Anti-miR-134 transfection plus 100nM RA treatment, 72hr, replicate 1 |
| GSM104070 |
100nM Anti-miR-134 transfection plus 100nM RA treatment, 72hr, replicate 2 |
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| Relations |
| BioProject |
PRJNA94609 |
| Supplementary data files not provided |
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