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| Status |
Public on Feb 01, 2009 |
| Title |
Gcn4p-mediated transcriptional stress response |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
The transcriptional data from an integrative analysis of transcriptional and metabolic stress responses that provides a more complete understanding of the mechanisms by which genetic regulatory circuits mediate metabolic phenotype.
Keywords: stress response, genetic modification
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| Overall design |
Our investigation disrupts native transcriptional control of amino acid biosynthesis via the Gcn4p- mediated transcriptional stress response. Specifically, genetic perturbation of transcriptional regulator Gcn4p in a stress condition is achieved via wild-type S288C and gcn4-delta strains in a pH-controlled chemostat environment in which a titrated inhibitor creates near histidine starvation and glucose supply limits growth to a pre-defined rate (0.10 hr^-1). The gene expression chip titles indicate the condition.
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| Contributor(s) |
Moxley JF, Jewett MC, Villas-Boas SG, Antoniewicz MR, Alper HS, Wheeler RT, Stephanopoulos G, Hinnebusch AG, Ideker T, Nielsen J, Stephanopoulos G |
| Citation(s) |
19346491 |
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| Submission date |
Apr 24, 2006 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Joel Moxley |
| E-mail(s) |
jfmoxley@mit.edu
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| Organization name |
MIT
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| Street address |
77 Massachusettes Ave
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (6)
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| GSM106371 |
wild type, YNB + 10 mM 3AT, replicate 1 |
| GSM106372 |
wild type, YNB + 10 mM 3AT, replicate 2 |
| GSM106373 |
-gcn4, YNB + 0.1 mM 3AT, replicate 1 |
| GSM106374 |
-gcn4, YNB + 0.1 mM 3AT, replicate 2 |
| GSM106375 |
wild type, YNB + 10 mM 3AT, replicate 3 |
| GSM106376 |
-gcn4, YNB + 0.1 mM 3AT, replicate 3 |
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| Relations |
| BioProject |
PRJNA95591 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE4709_RAW.tar |
8.8 Mb |
(http)(custom) |
TAR (of CEL) |
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