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Series GSE5976 Query DataSets for GSE5976
Status Public on Jul 01, 2008
Title Mesp1-induced gene expression changes
Organism Mus musculus
Experiment type Expression profiling by array
Summary During gastrulation, cells of the prospective mesoderm ingress through the primitive streak and acquire fates based on complex spatial and temporal cues. Progenitors of the cardiogenic mesoderm are first found at E6.5 in the posterior lateral epiblast and subsequently migrate laterally and anteriorly to form the cardiac crescent at E7.5, when regionalized cell fates are first delineated . Lineage tracing and heterotopic transplantation studies suggest that precursors in the earliest heart field possess potential to generate myocardium, endocardium, and pericardium. The mechanisms by which inductive signals in the primitive streak effect the development of this pancardiac progenitor field, however, remain poorly understood
In mice, the earliest restricted progenitors of the cardiovascular system are marked by expression of the basic helix-loop-helix transcription factor, Mesp1. We therefore use microarray analysis to determine the early and intermediate effects of transiently forced Mesp1 expression during ES cell differentiation.
Keywords: time course
Overall design ES cells bearing a targeted, doxycycline inducible Mesp1 transgene were differentiated in the absence or presence of DKK1 from day 0-4, either with or without transient doxycycline treatment from day 2-4.
Contributor(s) Lindsley RC, Murphy KM
Citation(s) 18593559
Submission date Oct 05, 2006
Last update date Feb 11, 2019
Contact name Coleman Lindsley
Organization name Washington University School of Medicine
Department Pathology and Immunology
Lab Dr. Kenneth M. Murphy
Street address BOX 8118, 660 S. Euclid Ave.
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM139021 A2lox.mesp1 (day 3)
GSM139022 A2lox.mesp1 (day 3 + dox)
GSM139023 A2lox.mesp1 (day 3 + DKK1)
BioProject PRJNA97453

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Supplementary file Size Download File type/resource
GSE5976_RAW.tar 69.7 Mb (http)(custom) TAR (of CEL)

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