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| Status |
Public on Jul 01, 2008 |
| Title |
Mesp1-induced gene expression changes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
During gastrulation, cells of the prospective mesoderm ingress through the primitive streak and acquire fates based on complex spatial and temporal cues. Progenitors of the cardiogenic mesoderm are first found at E6.5 in the posterior lateral epiblast and subsequently migrate laterally and anteriorly to form the cardiac crescent at E7.5, when regionalized cell fates are first delineated . Lineage tracing and heterotopic transplantation studies suggest that precursors in the earliest heart field possess potential to generate myocardium, endocardium, and pericardium. The mechanisms by which inductive signals in the primitive streak effect the development of this pancardiac progenitor field, however, remain poorly understood
In mice, the earliest restricted progenitors of the cardiovascular system are marked by expression of the basic helix-loop-helix transcription factor, Mesp1. We therefore use microarray analysis to determine the early and intermediate effects of transiently forced Mesp1 expression during ES cell differentiation.
Keywords: time course
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| Overall design |
ES cells bearing a targeted, doxycycline inducible Mesp1 transgene were differentiated in the absence or presence of DKK1 from day 0-4, either with or without transient doxycycline treatment from day 2-4.
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| |
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| Contributor(s) |
Lindsley RC, Murphy KM |
| Citation(s) |
18593559 |
| |
| Submission date |
Oct 05, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Coleman Lindsley |
| E-mail(s) |
lindsley@wustl.edu
|
| Organization name |
Washington University School of Medicine
|
| Department |
Pathology and Immunology
|
| Lab |
Dr. Kenneth M. Murphy
|
| Street address |
BOX 8118, 660 S. Euclid Ave.
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA97453 |
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