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| Status |
Public on Oct 24, 2008 |
| Title |
Expression data from xylem pole pericycle cells of Arabidopsis roots undergoing lateral root initiation |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We used microarrays to detail the global programme of gene expression during this process.
Keywords: time course
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| Overall design |
Arabidopsis xylem pole pericycle cells were isolated at successive stages of early lateral root development for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of cells at each stage in order to increase the temporal resolution of expression profiles. To that end, we used FACS on Arabidopsis roots containing a GFP marker for the xylem pole pericycle cells at three time-points: 1) arrest of G1-to-S transition (NPA or 0h NAA), 2) auxin signaling and progression through G1-to-S (2h NAA) and 3) G2-to-M transition or asymmetric cell division (6h NAA).
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| Contributor(s) |
De Smet I, Beeckman T, Benfey PN |
| Citation(s) |
18948541, 18622388 |
| |
| Submission date |
Nov 22, 2006 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Ive De Smet |
| E-mail(s) |
ive.desmet@zmbp.uni-tuebingen.de
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| Organization name |
University of Tübingen - Center for Plant Molecular Biology
|
| Street address |
Auf der Morgenstelle 3
|
| City |
Tuebingen |
| ZIP/Postal code |
D-72076 |
| Country |
Germany |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (6)
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| GSM146818 |
Xylem Pole Pericycle Cells at T0, biological rep1 |
| GSM146819 |
Xylem Pole Pericycle Cells at T0, biological rep2 |
| GSM146820 |
Xylem Pole Pericycle Cells at T2, biological rep1 |
| GSM146821 |
Xylem Pole Pericycle Cells at T2, biological rep2 |
| GSM146822 |
Xylem Pole Pericycle Cells at T6, biological rep1 |
| GSM146823 |
Xylem Pole Pericycle Cells at T6, biological rep2 |
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| Relations |
| BioProject |
PRJNA99617 |
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