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Series GSE6349 Query DataSets for GSE6349
Status Public on Oct 24, 2008
Title Expression data from xylem pole pericycle cells of Arabidopsis roots undergoing lateral root initiation
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary We used microarrays to detail the global programme of gene expression during this process.
Keywords: time course
Overall design Arabidopsis xylem pole pericycle cells were isolated at successive stages of early lateral root development for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of cells at each stage in order to increase the temporal resolution of expression profiles. To that end, we used FACS on Arabidopsis roots containing a GFP marker for the xylem pole pericycle cells at three time-points: 1) arrest of G1-to-S transition (NPA or 0h NAA), 2) auxin signaling and progression through G1-to-S (2h NAA) and 3) G2-to-M transition or asymmetric cell division (6h NAA).
Contributor(s) De Smet I, Beeckman T, Benfey PN
Citation(s) 18948541, 18622388
Submission date Nov 22, 2006
Last update date Jun 12, 2017
Contact name Ive De Smet
Organization name University of Tübingen - Center for Plant Molecular Biology
Street address Auf der Morgenstelle 3
City Tuebingen
ZIP/Postal code D-72076
Country Germany
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM146818 Xylem Pole Pericycle Cells at T0, biological rep1
GSM146819 Xylem Pole Pericycle Cells at T0, biological rep2
GSM146820 Xylem Pole Pericycle Cells at T2, biological rep1
BioProject PRJNA99617

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6349_RAW.tar 3.5 Mb (http)(custom) TAR (of CEL, TXT)

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