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Series GSE71811 Query DataSets for GSE71811
Status Public on Aug 10, 2016
Title Gene expression data from mouse regulatory T cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Intravenous Immunoglobulin (IVIg) is widely used as an immunomodulatory therapy. We have recently demonstrated that IVIg protects against airway hyper-reactivity (AHR) and inflammation in mouse models of allergic airway disease (AAD), associated with induction of Foxp3+ regulatory T cells (Treg). Using DEREG (DEpletion of REGulatory T cell) mice, in which endogenous Treg can be ablated with Diphtheria toxin (DTx) treatment, we demonstrate that IVIg generates a de novo population of induced Treg (iTreg) in the absence of endogenous Treg. IVIg-generated iTreg were sufficient for inhibition of ovalbumin-induced AHR in an antigen-driven murine model of AAD. In the absence of endogenous Treg, IVIg failed to confer protection against AHR and airway inflammation. Adoptive transfer of purified IVIg-generated iTreg prior to antigen challenge effectively prevented airway inflammation and AHR in an antigen-specific manner.
The goal of this study was to characterize the gene expression profile of a pure population of IVIg-generated induced Treg (iTreg).
Overall design Treg were isolated from mice sensitized and challenged with ovalbumin (OVA). Animals were treated with IVIg to generate iTreg with or without DTx endogenous Treg pre-depletion. Human serum albumin (HSA) was used as a control protein treatment.
RNA was isolated from 4 biological replicates for each condition. 12 samples in total were hybridized to Affymetrix gene expression microarrays.
variable: treatment: OVA-HSA-OVA - DTx: BMR133, BMR134, BMR135, BMR136
variable: treatment: OVA-IVIg-OVA - DTx: BMR137, BMR138, BR139, BMR140
variable: treatment: OVA-IVIg-OVA + DTx: BMR141, BMR142, BMR143, BMR144
repeat: biological replicate: eTreg: BMR133, BMR134, BMR135, BMR136
repeat: biological replicate: e_iTreg: BMR137, BMR138, BR139, BMR140
repeat: biological replicate: IVIg-iTreg: BMR141, BMR142, BMR143, BMR144
Contributor(s) Kaufman GN, Mazer BD
Citation(s) 28219891
Submission date Aug 06, 2015
Last update date Feb 21, 2018
Contact name Bruce David Mazer
Organization name RI-MUHC
Department RESP
Street address 1001 boul. D├ęcarie
City Montreal
State/province Quebec
ZIP/Postal code H4A 3J1
Country Canada
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (12)
GSM1846244 eTreg_rep1
GSM1846245 eTreg_rep2
GSM1846246 eTreg_rep3
BioProject PRJNA292115

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Supplementary file Size Download File type/resource
GSE71811_RAW.tar 112.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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