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Sample GSM1846253 Query DataSets for GSM1846253
Status Public on Aug 10, 2016
Title iTreg_rep2
Sample type RNA
Source name Treg, IVIg-induced
Organism Mus musculus
Characteristics tissue: spleen
cell type: T lymphocyte
Treatment protocol Spleens were dissected, lymphocytes were prepared, and Treg were isolated by flow cytometric sorting.
Growth protocol DEREG mice were sensitized and challenged with ovalbumin (OVA) to induce allergic airways disease. Endogenous Treg were depleted with injection of 40 ng/g diptheria toxin before IVIg or HSA treatment (2 g/kg).
Extracted molecule total RNA
Extraction protocol RNA was extracted with TRIzol reagent (Life Technologies) and cleaned with RNeasy Mini silica-gel membrane columns (Qiagen).
Label biotin
Label protocol 2 ng of total RNA were subjected to two rounds of amplification using Affymetrix GeneChip Pico WT two-cycle target labeling and control reagents according to the manufacturer's protocols
Hybridization protocol Following fragmentation, target ss-DNA was hybridized for 16 hours at 45°C to Affymetrix Mouse Gene 2.0 ST arrays. Arrays were stained and washed using the GeneChips Fluidics Station 450.
Scan protocol Arrays were scanned on an Affymetrix GeneChip Scanner 3000 7G. Images were processed using GeneChip Command Console (AGCC) software to generate CEL files.
Description Gene expression data of IVIg-generated induced Treg from allergen-exposed IVIg-treated animals
Data processing Raw data was read using the Bioconductor oligo package. Probe intensities were normalized across all arrays by the robust multi-array average (RMA) algorithm.
Submission date Aug 06, 2015
Last update date Aug 10, 2016
Contact name Bruce David Mazer
Organization name RI-MUHC
Department RESP
Street address 1001 boul. Décarie
City Montreal
State/province Quebec
ZIP/Postal code H4A 3J1
Country Canada
Platform ID GPL16570
Series (1)
GSE71811 Gene expression data from mouse regulatory T cells

Data table header descriptions
VALUE log(2) RMA-normalized signal intensity

Data table
17200001 6.621646122
17200003 7.255266242
17200005 5.41860593
17200007 6.437618555
17200009 7.322037364
17200011 7.295435932
17200013 6.90797194
17200015 8.098312662
17200017 6.01143715
17200019 6.831314431
17200021 6.749929728
17200023 8.203519754
17200025 7.668758278
17200027 6.732743321
17200029 3.558036538
17200031 4.480418765
17200033 5.073920784
17200035 6.879715663
17200037 8.22815867
17200039 4.172375466

Total number of rows: 41345

Table truncated, full table size 843 Kbytes.

Supplementary file Size Download File type/resource
GSM1846253_OVA-IVIg-OVA_+_DTx_002_BMR141-3_01.CEL.gz 9.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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