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| Status |
Public on Aug 15, 2007 |
| Title |
WT (Fis+) strain |
| Platform organism |
Escherichia coli K-12 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
|
| Summary |
Fis is a nucleoid-associated protein in E. coli that is abundant during early logarithmic growth in rich medium but is in short supply during stationary phase. Its role as a transcriptional regulator has been demonstrated for an increasing number of genes. In order to gain insight into the global effects of Fis on E. coli gene expression during different stages of growth in rich medium, DNA microarray analyses were conducted in fis and wild type strains during early log, mid log, late log, and stationary growth phases.
We used microarrays to detail the global impact of Fis on gene expression in Escherichia coli
Keywords: time course
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| Overall design |
E.coli cells were were grown and samples were taken at different times during growth: 90 min (early logarithmic phase), 150 min (mid-logarithmic phase), 240 min (late logarithmic phase), and 360 min (early stationary phase) for RNA extraction and hybridization on Affymetrix microarrays. This was done in triplicate and the raw data was analyzed using Microarray Analysis Suite version 5.0 (Affymetrix).
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| Contributor(s) |
Osuna R, Bradley MD, Beach MB, Pratt TS |
| Citation(s) |
17768236 |
| Submission date |
Mar 27, 2007 |
| Last update date |
Jul 26, 2013 |
| Contact name |
Robert Osuna |
| E-mail(s) |
osuna@albany.edu
|
| Phone |
518-591-8827
|
| Fax |
518-442-4767
|
| Organization name |
University at Albany
|
| Department |
Biology
|
| Lab |
Dr. Osuna
|
| Street address |
1400 Washington Avenue
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL199 |
[Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE7398 |
Expression data from E. coli covering four phases of growth in Fis KO and WT (Fis+) strains |
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| Relations |
| BioProject |
PRJNA104085 |
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