|
| Status |
Public on Aug 15, 2007 |
| Title |
wt 150 minutes growth rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Escherichia coli MG1655 wt grown 150 minutes
|
| Organism |
Escherichia coli |
| Characteristics |
wildtype Escherichia coli
150 minutes growth in LB
|
| Treatment protocol |
Cells were grown in LB at 37 degrees celsius with shaking for 150 minutes
|
| Growth protocol |
Saturated E. coli cultures were diluted in LB toan OD.of 0.05 OD600, grown at 37 degrees Celsius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MasterPure RNA purification Kit per manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip E.coli antisense. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from MG1655 wildtype after 150 minutes of growth in LB medium
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 27, 2007 |
| Last update date |
Mar 29, 2007 |
| Contact name |
Robert Osuna |
| E-mail(s) |
osuna@albany.edu
|
| Phone |
518-591-8827
|
| Fax |
518-442-4767
|
| Organization name |
University at Albany
|
| Department |
Biology
|
| Lab |
Dr. Osuna
|
| Street address |
1400 Washington Avenue
|
| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
| |
|
| Platform ID |
GPL199 |
| Series (2) |
| GSE7380 |
WT (Fis+) strain |
| GSE7398 |
Expression data from E. coli covering four phases of growth in Fis KO and WT (Fis+) strains |
|
