|
Status |
Public on Feb 25, 2016 |
Title |
Gene expression data for MACS-isolated primary splenic T cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
We are interested in unveiling potential gene expression alterations underlying the T cell-related phenotypes observed in DUSP4-/- mice. We used microarray to obtain a macroscopic views of gene profiles comparing freshly isolated splenic T cells from WT and DUSP4-/- mice
|
|
|
Overall design |
Magnetic-assisted cell sorting isolating T cells to 90-95% purity from freshly prepared splenocytes, followed by immediate RNA extraction.
|
Web link |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145880
|
|
|
Contributor(s) |
Huang C |
Citation(s) |
26710253 |
|
Submission date |
Nov 23, 2015 |
Last update date |
Feb 21, 2018 |
Contact name |
Ching-Yu Huang |
E-mail(s) |
cyhuang@nhri.org.tw
|
Organization name |
National Health Research Institutes
|
Department |
Immunology Research Center
|
Lab |
Huang Laboratory
|
Street address |
NO. 35, Keyan Rd.,
|
City |
Zhunan |
State/province |
Miao-Li |
ZIP/Postal code |
350 |
Country |
Taiwan |
|
|
Platforms (1) |
GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA303176 |