|
| Status |
Public on Feb 25, 2016 |
| Title |
DUSP4-T-2 |
| Sample type |
RNA |
| |
|
| Source name |
MACS-sorted splenic T cell sample DUSP4 KO 2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
genotype: dusp4 ko
age: 6-8 wk old
|
| Treatment protocol |
n/a; primary cells isolated via MACS methods without treatment.
|
| Growth protocol |
n/a; primary cells isolated via MACS methods without culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 5.5 ug fragmentation cDNA
|
| |
|
| Hybridization protocol |
3.5 ug of labelling cDNA were hybridized for 16 hr at 45C on GeneChip (MoGene-2.0-st). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip scanner 3000 7G (Affymatrix), GeneChip® Command Console® Software.
|
| Description |
Gene expression data from MACS-sorted splenic T cell sample DUSP4 KO 2
|
| Data processing |
The data were analyzed using Affymetrix Expression Console™ Software (version 1.4) default analysis settings and RMA (Robust Multi-Array) summarization and normalization options.
|
| |
|
| Submission date |
Nov 23, 2015 |
| Last update date |
Feb 25, 2016 |
| Contact name |
Ching-Yu Huang |
| E-mail(s) |
cyhuang@nhri.org.tw
|
| Organization name |
National Health Research Institutes
|
| Department |
Immunology Research Center
|
| Lab |
Huang Laboratory
|
| Street address |
NO. 35, Keyan Rd.,
|
| City |
Zhunan |
| State/province |
Miao-Li |
| ZIP/Postal code |
350 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE75319 |
Gene expression data for MACS-isolated primary splenic T cells |
|
