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| Status |
Public on Dec 21, 2017 |
| Title |
RNA-seq to identify P-body associated mRNAs upon glucose starvation, Na+ and Ca2+ stresses |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To examine mRNA species sequestered by P-bodies upon different stress conditions, we employed a modified crosslinking immunoprecipitation (cCLAP) technique to purify P-body associated mRNAs and analyzed by RNA-Seq. To compare, we also performed total RNA-Seq under the same stress conditions.
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| Overall design |
five biological independent replicates for P-body associated RNA-Seq, each consists of four conditions (control, glucose starvation, Na+and Ca2+) and three biological independent replicates for total RNA-Seq, each consists of four conditions (control, glucose starvation, Na+and Ca2+).
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| Contributor(s) |
Wang C, Schmich F, Srivatsa S |
| Citation(s) |
29297464 |
| Submission date |
Dec 31, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Anne Spang |
| Organization name |
Biozentrum, University of Basel
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| Street address |
Klingelbergstr.50/70
|
| City |
Basel |
| ZIP/Postal code |
4056 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA307668 |
| SRA |
SRP068102 |
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