NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE77102 Query DataSets for GSE77102
Status Public on Apr 01, 2016
Title Analysis of transcriptional signatures in response to Listeria monocytogenes infection reveals temporal and strain dependent changes in interferon signalling
Organism Mus musculus
Experiment type Expression profiling by array
Summary Listeriosis is an infectious disease caused by the intracellular bacterium Listeria monocytogenes. To control the infection effectively, the host immune response is directed by intercellular signalling molecules called cytokines that are produced by immune cells following sensing of the bacteria. In this study we used gene expression analysis to examine complex immune signalling networks in the blood and tissues of mice infected with L. monocytogenes. We show that a large set of genes are perturbed in both blood and tissue upon infection and that the transcriptional responses in both are enriched for pathways of the immune response. From these data we also observe an important signalling network emerge from a group of cytokines called interferons (IFNs). Previous findings suggest that different IFN family members can determine the balance between successful and impaired immune responses to L. monocytogenes and several other bacterial infections. Using mice deficient for the detrimental ‘type I’ IFN signalling pathway we show that IFN-inducible genes are differentially regulated at different times upon infection but also present at much lower levels in uninfected mice highlighting how dysregulation of this network in the steady state may determine the outcome of this bacterial infection.
 
Overall design Total RNA obtained from isolated blood, liver and spleen of C57BL/6 and B6 IFNAR-/- mice subjected to acute infection by Listeria monocytogenes compared to uninfected controls.
 
Contributor(s) Pitt JM, Blankley S, Potempa K, Graham CM, McNab FW, Moreira-Teixeira L, Howes A, Stavropoulos E, Pascual V, Banchereau J, Chaussabel D, O'Garra A
Citation(s) 26918359
Submission date Jan 21, 2016
Last update date Jan 16, 2019
Contact name Krzysztof Potempa
E-mail(s) potempak@gmail.com
Organization name National Institute for Medical Research (NIMR)
Street address The Ridgeway
City London
ZIP/Postal code NW7 1AA
Country United Kingdom
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (192)
GSM2044171 Blood Infected_Day 3 rep 1_experiment_1
GSM2044172 Blood Infected_Day 3 rep 2_experiment_1
GSM2044173 Blood Infected_Day 3 rep 3_experiment_1
Relations
BioProject PRJNA309435

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE77102_Non-normalized_data.txt.gz 31.0 Mb (ftp)(http) TXT
GSE77102_RAW.tar 15.8 Mb (http)(custom) TAR
Processed data included within Sample table
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap