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Status |
Public on Oct 06, 2016 |
Title |
Pathogenicity of genomic duplications is determined by formation of novel chromatin domains (neo-TADs) (4C-seq) |
Organisms |
Homo sapiens; Mus musculus |
Experiment type |
Other
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Summary |
Genome-scale methods have identified subchromosomal structures so-called topologically associated domains (TADs) that subdivide the genome into discrete regulatory units, establish with their target genes. By re-engineering human duplications at the SOX9 locus in mice combined with 4C-seq and Capture Hi-C experiments, we show that genomic duplications can result in the formation of novel chromatin domains (neo-TADs) and that this process determines their molecular pathology.
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Overall design |
Circular Chromosome Conformation Capture (4C seq) and Capture Hi-C experimetns at the Sox9/Kcnj locus in developing distal limbs of mutants and WT animals at E12.5 as well as from patient fibroblasts
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Contributor(s) |
Ibrahim DM, Franke M, Schöpflin R, Heinrich V, Andrey G, Mundlos S |
Citation(s) |
27706140 |
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Submission date |
Feb 18, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Daniel Murad Ibrahim |
E-mail(s) |
ibrahim@molgen.mpg.de, daniel.ibrahim@bih-charite.de
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Phone |
030 84131516
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Organization name |
Berlin Institute of Health
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Department |
Center for Regenerative Therapies
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Street address |
Augustenburger Platz 1
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City |
Berlin |
State/province |
Berlin |
ZIP/Postal code |
13353 |
Country |
Germany |
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Platforms (4)
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GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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Samples (25)
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This SubSeries is part of SuperSeries: |
GSE78109 |
Pathogenicity of genomic duplications is determined by formation of novel chromatin domains (neo-TADs) |
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Relations |
BioProject |
PRJNA312529 |
SRA |
SRP070527 |