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Series GSE79212 Query DataSets for GSE79212
Status Public on Jun 12, 2017
Title Gene expression analysis in wild-type and OsHOX24 rice overexpression line under control and drought stress conditions
Organism Oryza sativa
Experiment type Expression profiling by array
Summary Several homeobox genes belonging to HD-ZIP I subfamily are highly induced by drought stress at various developmental stages in rice. To analyze the role of a candidate HD-ZIP I subfamily member, OsHOX24, we constitutively overexpressed it in rice. The physiological analyses revealed that overexpression of OsHOX24 gene reduced drought stress tolerance in transgenic plants as compared to wild-type.
We used microarrays to study the global effect of OsHOX24 overexpression in rice as compared to wild-type under control and drought stress condition.
Overall design Three-weeks-old light-grown wild-type and transgenic rice seedlings grown under controlled conditions and those subjected to drought stress treatment were used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates of each sample were used for microarray analysis. For drought stress treatment (DS), rice seedlings were dried for 3 h between folds of tissue paper at 28±1 degree C, in a culture room. The seedlings kept in water for 3 h, at 28±1 degree C, served as control (seedling).
Contributor(s) Bhattacharjee A, Jain M
Citation(s) 28484484
Submission date Mar 14, 2016
Last update date Mar 08, 2018
Contact name Mukesh Jain
Phone 91-11-26704686
Organization name Jawaharlal Nehru University
Street address New Mehrauli Road
City New Delhi
State/province New Delhi
ZIP/Postal code 110067
Country India
Platforms (1)
GPL21593 [RUSGene-1_0-st] Affymetrix Rice (US) Gene 1.0 ST Array [transcript (gene) version]
Samples (12)
GSM2088150 Wild-type seedlings, control, rep1
GSM2088151 Wild-type seedlings, control, rep2
GSM2088152 Wild-type seedlings, control, rep3
BioProject PRJNA315162

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Supplementary file Size Download File type/resource
GSE79212_RAW.tar 55.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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