|
| Status |
Public on Jun 12, 2017 |
| Title |
OsHOX24-overexpression seedlings, drought stress, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Rice seedlings
|
| Organism |
Oryza sativa |
| Characteristics |
genotype/variation: OsHOX24 overexpressed
stress: drought
ecotype: Pusa Basmati 1
tissue: Seedlings
age: Three week old
|
| Treatment protocol |
For control conditions and drought stress treatment, seedlings were kept in RO water and dried between folds of tissue paper at 28 ± 1 degree celsius for 3 h, respectively
|
| Growth protocol |
Wild-type and transgenic rice seedlings were grown in hydroponics system (trays) containing Yoshida medium, at 28±1 degree celsius for three weeks, in a culture room with a daily photoperiodic cycle of 14 h light and 10 h dark
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using Trizol, according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 500 ng total RNA (GeneChip® 3' IVT PLUS Reagent Kit User Manual, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.5 ug of labelled 2nd cycle ss-cDNA were hybridized for 17 h at 45 degree celsius on GeneChip whole target expression array. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450 as per FS450_0002 fluidics protocol.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000
|
| Description |
Gene expression data from drought stress treated OsHOX24 overexpression rice seedlings
|
| Data processing |
CEL files were imported into Genespring software and probe summarization and normalization was performed using RMA algorithm
|
| |
|
| Submission date |
Mar 14, 2016 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Mukesh Jain |
| Phone |
91-11-26704686
|
| Organization name |
Jawaharlal Nehru University
|
| Street address |
New Mehrauli Road
|
| City |
New Delhi |
| State/province |
New Delhi |
| ZIP/Postal code |
110067 |
| Country |
India |
| |
|
| Platform ID |
GPL21593 |
| Series (1) |
| GSE79212 |
Gene expression analysis in wild-type and OsHOX24 rice overexpression line under control and drought stress conditions |
|
