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| Status |
Public on Jun 05, 2007 |
| Title |
Common genome-wide patterns of transcript accumulation underlying the wing polyphenism and polymorphism in the pea aphid |
| Platform organisms |
Buchnera aphidicola; Escherichia coli; Acyrthosiphon pisum |
| Sample organism |
Acyrthosiphon pisum |
| Experiment type |
Expression profiling by array
|
| Summary |
The pea aphid, Acyrthosiphon pisum, exhibits several environmentally cued polyphenisms, in which discrete, alternative phenotypes are produced. At low density parthenogenetic females produce unwinged female progeny, but at high density females produce progeny that develop with wings. These alternative phenotypes represent a solution to the competing demands of dispersal and reproduction. Males also develop as either winged or unwinged, but these alternatives are determined by a genetic polymorphism. Winged and unwinged males are morphologically less distinct from each other than winged and unwinged females, possibly because males experience fewer trade-offs between dispersal and reproduction. To assess whether shared physiological differences mirror the shared morphological differences that characterize the wing polyphenism and polymorphism, we used a cDNA microarray representing an estimated 10% of the coding genome (1734 genes) to examine differential transcript accumulation between winged and unwinged females and males. We identified several transcripts that differentially accumulate between winged and unwinged morphs in both sexes, the majority of which are involved in energy production. Unexpectedly, the extent of differential transcript accumulation between winged and unwinged morphs was greater for adult males than for adult females. Together, these results suggest not only that similar physiological differences underlie the polyphenism and polymorphism, but that male morphs, like females, are subject to trade-offs between reproduction and dispersal that are reflected in levels of transcript accumulation and possibly genome-wide patterns of gene regulation. These data also provide a baseline for future studies of the molecular and physiological basis of life history trade-offs.
Keywords: Transcript levels were compared between winged and unwinged male and female pea aphids, for both nymphs and adults.
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| Overall design |
Labeled cDNA made from RNA isolated from winged and unwinged male and female pea aphids (nymphs and adults) was hybridized to a spotted cDNA microarray (1734 aphid genes) against a common referent. Three biological replicates of each aphid type (winged state, sex, stage) were performed.
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| Contributor(s) |
Brisson JA, Davis GK, Stern DL |
| Citation(s) |
17651358 |
| |
| Submission date |
Jun 04, 2007 |
| Last update date |
Apr 24, 2013 |
| Contact name |
Gregory K. Davis |
| E-mail(s) |
gkdavis@princeton.edu
|
| Phone |
609-258-5146
|
| URL |
http://www.princeton.edu/~gkdavis/
|
| Organization name |
Princeton University
|
| Department |
Ecology and Evolutionary Biology
|
| Lab |
Stern Lab
|
| Street address |
106A Guyot Hall, Washington Road
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
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| Platforms (1) |
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| Samples (24)
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| GSM197778 |
Unwinged 5th Instar Females Replicate 1 |
| GSM197779 |
Winged 5th Instar Females Replicate 1 |
| GSM197780 |
Unwinged 5th Instar Males Replicate 1 |
| GSM197781 |
Winged 5th Instar Males Replicate 1 |
| GSM197782 |
Unwinged 4th Instar Females Replicate 1 |
| GSM197783 |
Winged 4th Instar Females Replicate 1 |
| GSM197784 |
Unwinged 4th Instar Males Replicate 1 |
| GSM197785 |
Winged 4th Instar Males Replicate 1 |
| GSM197786 |
Unwinged 5th Instar Females Replicate 2 |
| GSM197787 |
Winged 5th Instar Males Replicate 2 |
| GSM197788 |
Winged 4th Instar Females Replicate 2 |
| GSM197789 |
Winged 4th Instar Females Replicate 3 |
| GSM197790 |
Unwinged 4th Instar Females Replicate 2 |
| GSM197791 |
Winged 5th Instar Males Replicate 3 |
| GSM197792 |
Winged 5th Instar Females Replicate 2 |
| GSM197793 |
Unwinged 5th Instar Females Replicate 3 |
| GSM197794 |
Winged 4th Instar Males Replicate 2 |
| GSM197795 |
Winged 5th Instar Females Replicate 3 |
| GSM197796 |
Unwinged 5th Instar Males Replicate 2 |
| GSM197797 |
Unwinged 4th Instar Males Replicate 2 |
| GSM197798 |
Unwinged 5th Instar Males Replicate 3 |
| GSM197799 |
Unwinged 4th Instar Males Replicate 3 |
| GSM197800 |
Winged 4th Instar Males Replicate 3 |
| GSM197801 |
Unwinged 4th Instar Females Replicate 3 |
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| Relations |
| BioProject |
PRJNA100799 |
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