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| Status |
Public on Jun 29, 2016 |
| Title |
Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression regulation. To study lncRNAs in cancer, we generated expression profiles for 1708 human lncRNAs in the NCI60 cancer cell line panel using a high-throughput nanowell RT-qPCR platform. We describe how qPCR assays were designed and validated and provide processed and normalized expression data for further analysis. Data quality is demonstrated by matching the lncRNA expression profiles with phenotypic and genomic characteristics of the cancer cell lines. This data set can be integrated with publicly available omics and pharmacological data sets to uncover novel associations between lncRNA expression and mRNA expression, miRNA expression, DNA copy number, protein coding gene mutation status or drug response.
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| |
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| Overall design |
lncRNA expression profiling of 60 cancer cell lines
|
| |
|
| Contributor(s) |
Mestdagh P, Vandesompele J |
| Citation(s) |
27377824 |
| Submission date |
Apr 15, 2016 |
| Last update date |
Jul 07, 2016 |
| Contact name |
Pieter mestdagh |
| E-mail(s) |
pieter.mestdagh@ugent.be
|
| Organization name |
Center for Medical Genetics
|
| Street address |
De Pintelaan 185
|
| City |
Gent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL21738 |
Wafergen Biosystems SmartChip Human lncRNA panel |
|
| Samples (60)
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|
| Relations |
| BioProject |
PRJNA318621 |
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