cell pellets were obtained from the National Cancer Institute (Developmental Therapeutics Program)
Extracted molecule
total RNA
Extraction protocol
RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer's instructions.
Label
SYBR Green
Label protocol
Labeling with SYBR Green
Hybridization protocol
n/a
Scan protocol
n/a
Description
SAMPLE 4
Data processing
Raw Cq-values were filtered based on a detection threshold of 28 PCR cycles, excluding measurements with Cq > 28. The median Cq-value of triplicate measurements per gene was subsequently used to calculate a sample-specific normalization factor, applying the global mean normalization strategy. Briefly, each Cq-value was normalized by subtracting the mean Cq-value per sample. Normalized Cq-values were multiplied by -1 such that higher values represent higher expression levels.
