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Series GSE90032 Query DataSets for GSE90032
Status Public on Apr 18, 2018
Title Srf destabilizes cell identity (Microarray_affymetrix)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Multicellular organisms consist of multiple cell types, whose identities are maintained appropriately at locations where they are reside. The identity of each cell type is primarily maintained by cell-type-specific gene expression programs, but mechanisms that suppress these programs are poorly defined. Here we show that serum response factor (Srf), a transcription factor that is activated by various extracellular stimuli, can repress cell-type-specific genes and promote cellular reprogramming to pluripotency. Manipulations that decrease β-actin monomer resulted in nuclear accumulation of Mkl1 and the activation of Srf, which downregulated cell-type-specific genes and altered epigenetics in enhancers and chromatin organization. Mice overexpressing Srf exhibited various pathologies including an ulcerative colitis-like symptom and a metaplasia-like phenotype in the pancreas. Our results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases.
 
Overall design Total RNAs of hepatoblasts expressing EGFP and overexpressing Srf were used to analyze effects of overexpressions of Srf on changes of gene expressions.
 
Contributor(s) Ikeda T, Hikichi T, Yamamoto T, Okita K, Masui S
Citation(s) 29643333
Submission date Nov 18, 2016
Last update date Jul 18, 2018
Contact name Takashi Ikeda
E-mail(s) tikeda@cira.kyoto-u.ac.jp
Organization name Kyoto Univ.
Street address 53 Shogoin Kawahara-cho Sakyo-ku
City Kyoto
ZIP/Postal code 6068507
Country Japan
 
Platforms (1)
GPL16570 [MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version]
Samples (2)
GSM2830360 Hep_EGFP
GSM2830361 Hep_Srf
This SubSeries is part of SuperSeries:
GSE90034 Srf destabilizes cell identity
Relations
BioProject PRJNA354544

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE90032_RAW.tar 18.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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