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| Status |
Public on Apr 18, 2018 |
| Title |
Srf destabilizes cell identity (Microarray_affymetrix) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Multicellular organisms consist of multiple cell types, whose identities are maintained appropriately at locations where they are reside. The identity of each cell type is primarily maintained by cell-type-specific gene expression programs, but mechanisms that suppress these programs are poorly defined. Here we show that serum response factor (Srf), a transcription factor that is activated by various extracellular stimuli, can repress cell-type-specific genes and promote cellular reprogramming to pluripotency. Manipulations that decrease β-actin monomer resulted in nuclear accumulation of Mkl1 and the activation of Srf, which downregulated cell-type-specific genes and altered epigenetics in enhancers and chromatin organization. Mice overexpressing Srf exhibited various pathologies including an ulcerative colitis-like symptom and a metaplasia-like phenotype in the pancreas. Our results demonstrate an unexpected function of Srf via a mechanism by which extracellular stimuli actively destabilize cell identity and suggest Srf involvement in a wide range of diseases.
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| Overall design |
Total RNAs of hepatoblasts expressing EGFP and overexpressing Srf were used to analyze effects of overexpressions of Srf on changes of gene expressions.
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| Contributor(s) |
Ikeda T, Hikichi T, Yamamoto T, Okita K, Masui S |
| Citation(s) |
29643333 |
| |
| Submission date |
Nov 18, 2016 |
| Last update date |
Jul 18, 2018 |
| Contact name |
Takashi Ikeda |
| E-mail(s) |
tikeda@cira.kyoto-u.ac.jp
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| Organization name |
Kyoto Univ.
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| Street address |
53 Shogoin Kawahara-cho Sakyo-ku
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| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA354544 |
