|
| Status |
Public on Apr 18, 2018 |
| Title |
Hep_Srf |
| Sample type |
RNA |
| |
|
| Source name |
Hepatoblasts, Srf overexpression
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Primary culture isolated from fetal liver
cell type: Hepatoblasts
|
| Treatment protocol |
The transgene of Srf was introduced retrovirally.
|
| Growth protocol |
Hepatoblasts were maintained on a type IV collagen-coated culture plate in a medium consisting of a 1:1 mixture of DMEM/Ham’s F-12 with L-glutamine, HEPES and sodium pyruvate (Wako) supplemented with 10% fetal bovine serum (vol/vol) (Nichirei Bioscience), 1 µg/ml insulin (Wako), 100 nM dexamethasone (Sigma-Aldrich), 10 mM nicotinamide (Sigma-Aldrich), 50 µM β-mercaptoethanol (Nacalai Tesque), 50 ng/ml human recombinant hepatocyte growth factor (HGF) (Peprotech) and 20 ng/ml epidermal growth factor (EGF) (Peprotech).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) and was assessed by Agilent 2100 Bioanalyzer and the RNA 6000 LabChip Kit (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Transcriptional amplification were performed using 200 ng of total RNA following the Whole Transcript (WT) Expression kit Ambion/Affymetrix).
|
| |
|
| Hybridization protocol |
The fragmented and biotin-labeled cDNA targets were hybridized to mouse Gene 2.0 ST arrays (Affymetrix) according to Affymetrix protocols.
|
| Scan protocol |
Hybridized arrays were scanned using an Affymetrix GeneChip Scanner.
|
| Description |
Srf overexpression
|
| Data processing |
The array data were analyzed using GeneSpring software. Gene expression values were normalized by excluding low-signal intensity data and percentile shifts.
|
| |
|
| Submission date |
Oct 25, 2017 |
| Last update date |
Apr 18, 2018 |
| Contact name |
Takashi Ikeda |
| E-mail(s) |
tikeda@cira.kyoto-u.ac.jp
|
| Organization name |
Kyoto Univ.
|
| Street address |
53 Shogoin Kawahara-cho Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE90032 |
Srf destabilizes cell identity (Microarray_affymetrix) |
| GSE90034 |
Srf destabilizes cell identity |
|
