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| Status |
Public on Jan 06, 2017 |
| Title |
mRNA profile in CD4+ T cell in healthy control and Primay biliary cholangitis (PBC) [mRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Primary biliary cholangitis (PBC), formally known as primary biliary cirrhosis, is an autoimmune liver disease of unknown pathogenesis. Consequently, therapeutic targets for PBC have yet to be identified. As CD4+ T cells play a pivotal role in immunological dysfunction observed in PBC, we analyzed microRNA(miRNA) and mRNA expression in CD4+ T cells to investigate PBC pathogenesis and identify novel therapeutic targets.
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| Overall design |
Clinically and pathologically diagnosed 6 PBC patients and 6 healthy controls, who agreed to provide samples with written informed consent, were enrolled in this study. Total RNA was extracted from peripheral CD4+ T cells from PBC patients and healthy controls. All samples fulfilled the following criteria (RNA integrity number > 8.0, A260/A280 ratio; 1.9-2.2) by Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and a DU730 UV/Visible Spectrophotometer (Beckman Coulter, Brea, CA, USA). All samples profiled the expression of miRNA and mRNA by using Agilent microarray (SurePrint G3 human miRNA microarray kit 8×60K Rel.16.0 and SurePrint G3 human GE 8×60K microarray kit) according to manufacturer’s protocols.
mRNA profiling was performed using Agilent gene expression array platforms with RNAs isolated from CD4+ T cell of PBC and healthy control.
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| Contributor(s) |
Kato N, Nakagawa R |
| Citation(s) |
28192189 |
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| Submission date |
Jan 05, 2017 |
| Last update date |
May 31, 2017 |
| Contact name |
Ryo Nakagawa |
| E-mail(s) |
ryo420@ims.u-tokyo.ac.jp
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| Organization name |
The University of Tokyo
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| Department |
The Institute of Medical Science
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| Lab |
Division of Advanced Genome Medicine
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| Street address |
4-6-1 Shirokanedai, Minato-ku
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| City |
Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
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| Platforms (1) |
| GPL14550 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Probe Name Version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE93172 |
microRNA-mRNA integral analysis in CD4+ T cell in primay biliary cholangitis (PBC) |
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| Relations |
| BioProject |
PRJNA360235 |
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