|
| Status |
Public on Jan 06, 2017 |
| Title |
PBC_2 |
| Sample type |
RNA |
| |
|
| Source name |
PBC
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Primay biliary cholangitis (PBC)
tissue: CD4+ Tcell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TotalRNA was extracted by miRNeasy mini kit ((Qiagen, Germantown, MD))
|
| Label |
Cy3
|
| Label protocol |
Total RNA (50 ng) was reverse transcribed and Cyanine3-pCp labeled using Low-input quick amp labelling kits, one color (Agilent Technologies) according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridezed using gene expression hybridisation kits (Agilent Technologies) according to the manufacturer’s protocol.
|
| Scan protocol |
Array images were scanned on Agilent’s G2505C scanner and Feature Extraction software 10.7 (Agilent Technologies) with default settings.
|
| Description |
Sample_8
|
| Data processing |
Raw data were normalized by 90the percentile algorithm, Gene Spring Software 12.5 (Agilent Technologies).
|
| |
|
| Submission date |
Jan 05, 2017 |
| Last update date |
Jan 06, 2017 |
| Contact name |
Ryo Nakagawa |
| E-mail(s) |
ryo420@ims.u-tokyo.ac.jp
|
| Organization name |
The University of Tokyo
|
| Department |
The Institute of Medical Science
|
| Lab |
Division of Advanced Genome Medicine
|
| Street address |
4-6-1 Shirokanedai, Minato-ku
|
| City |
Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (2) |
| GSE93170 |
mRNA profile in CD4+ T cell in healthy control and Primay biliary cholangitis (PBC) [mRNA] |
| GSE93172 |
microRNA-mRNA integral analysis in CD4+ T cell in primay biliary cholangitis (PBC) |
|
