|
| Status |
Public on Jul 07, 2008 |
| Title |
IRF4 ChIP-on-chip for Myeloma Cell Lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To identify IRF4 transcription factor binding on chromatin at 5' regulatory regions of genes in myeloma cell line models.
Keywords: binding site identification design
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| |
|
| Overall design |
Formaldehyde cross-linked, sonicated chromatin is prepared from cell lines Kms12 (test) and Ly19 (control). Chromatin immunoprecipated with anti-IRF4 antibody is labeled with Cy5 and co-hybridized on Agilent Human Promoter Set arrays with chromatin immunoprecipated with normal sera labeled with Cy3 . Two biological replicates were performed for the Kms12 and Ly19 experiments.
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| |
|
| Citation(s) |
18568025 |
| |
| Submission date |
Oct 18, 2007 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
|
| Phone |
301-402-1892
|
| Organization name |
National Cancer Institute
|
| Department |
Lymphoid Malignancies Branch
|
| Lab |
Louis M Staudt
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL4124 |
Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Feature Number version) |
| GPL4125 |
Agilent-014707 Human Promoter ChIP-on-Chip Set 244K, Microarray 2 of 2 G4489A (Feature Number version) |
|
| Samples (8)
|
| GSM238443 |
Kms12 with IRF4 - slide 1 - repeat 1 |
| GSM238444 |
Kms12 with IRF4 - slide 2 - repeat 1 |
| GSM238445 |
Ly19 with IRF4 - slide 1 - repeat 1 |
| GSM238446 |
Ly19 with IRF4 - slide 2 - repeat 1 |
| GSM238447 |
Kms12 with IRF4 - slide 1 - repeat 2 |
| GSM238448 |
Kms12 with IRF4 - slide 2 - repeat 2 |
| GSM238449 |
Ly19 with IRF4 - slide 1 - repeat 2 |
| GSM238450 |
Ly19 with IRF4 - slide 2 - repeat 2 |
|
| Relations |
| BioProject |
PRJNA103071 |
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