|
| Status |
Public on Jul 07, 2008 |
| Title |
Kms12 with IRF4 - slide 1 - repeat 1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Kms12 with IRF4 antibody
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line: Kms12
Cell type: myeloma cell line
|
| Treatment protocol |
Treatment type: compound
Agent: Anti-IRF4 Antibody
Treatment dose: 10 ug
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA Preparation Protocol
Other: Formaldehyde cross-linked, sonicated chromatin is prepared from cell lines Kms12 and Ly19 with chromatin immunoprecipitation protocol.
|
| Label |
cy5
|
| Label protocol |
Agilent Cy5 Labeling Protocol
Other: Whole genome amplified DNA is labeled using random primed Klenow enzyme based extension protocol according to Agilent's Mammalian ChIP-on-chip protocol v9.1.
|
| |
|
| Channel 2 |
| Source name |
Kms12 Control
|
| Organism |
Homo sapiens |
| Characteristics |
Cell line: Kms12
Cell type: myeloma cell line
|
| Treatment protocol |
Treatment type: compound
Agent: Normal goat serum
Treatment dose: 10 ug
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA Preparation Protocol
Other: Formaldehyde cross-linked, sonicated chromatin is prepared from cell lines Kms12 and Ly19 with chromatin immunoprecipitation protocol.
|
| Label |
cy3
|
| Label protocol |
Agilent Cy3 Labeling Protocol
Other: Whole genome amplified DNA is labeled using random primed Klenow enzyme based extension protocol according to Agilent's Mammalian ChIP-on-chip protocol v9.1.
|
| |
|
| |
| Hybridization protocol |
Agilent Hybridization Protocol
Other: Five micrograms of each Cy5 and Cy3 labeled DNA is hybridized to array for 40 hours at 65 degrees using Agilent ACGH/ChIp-chip hybridization and wash kits.
|
| Scan protocol |
Agilent Scanning Protocol
Other: Arrays were scanned at 5um resolution on an Agilent DNA Microarray Scanner (G2505B, Agilent) using the default settings for 244k format two-color arrays.
|
| Description |
No additional information.
|
| Data processing |
Agilent Data Processing Protocol
Calculation Method: Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction Software (Version 9.1.3.1). Spot values were normalized using the default normalization parameters.
|
| |
|
| Submission date |
Oct 18, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
|
| Phone |
301-402-1892
|
| Organization name |
National Cancer Institute
|
| Department |
Lymphoid Malignancies Branch
|
| Lab |
Louis M Staudt
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL4124 |
| Series (1) |
| GSE9367 |
IRF4 ChIP-on-chip for Myeloma Cell Lines |
|
