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| Status |
Public on Sep 11, 2020 |
| Title |
Wnt/planar cell polarity primed intestinal stem cells directly differentiate into enteroendocrine or Paneth cells [MoGene-2_0-st] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Imbalance in intestinal stem cell (ISC) homeostasis leads to cancer and metabolic disease. Therefore intense efforts are underway to understand ISC hierarchy and the niche signals that control stem cell fate. Several ISC populations have been described according to their marker expression, cell-cycle behavior and lineage potential. Actively cycling Lgr5+ ISCs ensure homeostatic renewal. A less well-defined and minor population of quiescent and label-retaining cells (LRCs) is viewed as a reserve stem cell pool. However, the existence of quiescent stem cells distinct from the Lgr5+ ISCs is controversial. Indeed, recent findings identified quiescent intestinal cells as a subpopulation of Lgr5+ ISCs that are committed to the secretory fate, but the signals that maintain and activate quiescent cells/LRCs remain elusive.
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| Overall design |
We compared intestinal stem cells expressing high levels of Lgr5 and GFP with Flattop-expressing intestinal crypt cells
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| Contributor(s) |
Böttcher A, Büttner M, Tritschler S, Sterr M, Aliluev A, Burtscher I, Sass S, Irmler M, Beckers J, Ziegenhain C, Enard W, Schamberger AC, Verhamme FM, Eickelberg O, Theis FJ, Lickert H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 26, 2017 |
| Last update date |
Sep 14, 2020 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
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| Organization name |
Helmholtz Zentrum Muenchen
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| Department |
Institute of Experimental Genetics
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| Street address |
Ingolstaedter Landstr. 1
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| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE94092 |
Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates |
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| Relations |
| BioProject |
PRJNA368851 |
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