|
| Status |
Public on Sep 11, 2020 |
| Title |
Lgr5_GFPhigh_01_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Intestinal stem cells expressing high levels of Lgr5 and GFP
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
age: 3-6 months
genotype: Lgr5-EGFP-IRES-creERT2
|
| Treatment protocol |
As described in the paper. Briefly, single cells were pepared and FACS-sorted
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Micro Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Ovation Pico WTA System V2 in combination with the Encore Biotin Module (Nugen)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol including minor modifications as suggested in the Encore Biotin protocol
|
| Scan protocol |
According to the Affymetrix expression protocol, Scanner 3000 7G
|
| Description |
Gene expression data from intestinal stem cells expressing high levels of Lgr5 and GFP
|
| Data processing |
RMA normalized using R/Bioconductor package oligo (version 1.38.0)
|
| |
|
| Submission date |
Jan 26, 2017 |
| Last update date |
Sep 11, 2020 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (2) |
| GSE94090 |
Wnt/planar cell polarity primed intestinal stem cells directly differentiate into enteroendocrine or Paneth cells [MoGene-2_0-st] |
| GSE94092 |
Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates |
|
