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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 13, 2007 |
| Title |
Gene profiling approaches help to define the specific functions of retinoblastoma family in epidermis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The epidermal-specific ablation of Rb gene leads to increased proliferation, aberrant differentiation, and the disengagement of these processes in vivo and in vitro. These differences in phenotype are more severe with the loss of p107, demonstrating the functional compensation between pRb and p107. As p107 and p130 also exert overlapping functions in epidermis, we have generated Rb(F19/F19)K14cre;Rbl2-/- (pRb-;p130-) mice to analyze possible functional redundancies between pRb and p130. The epidermal phenotype was very similar between pRb- and pRb-;p130- mice, suggesting that pRb and p130 activities are not redundant in epidermis. Importantly, we can correlate the proliferation differences with specific changes in gene expression between pRb-, pRb-;p107- and pRb-;p130- primary keratinocytes using microarray analysis, and explain the phenotypes in the context of altered E2F expression and functionality. Our findings support a model in which the distinct retinoblastoma family members, in conjunction with E2F members, play a central role in regulating epidermal homeostasis through specific or overlapping activities.
Keywords: skin, pRb, transgenic mice, p130
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| Overall design |
Total RNA from pooled cells from each genotype were analyzed, per duplicate (pRb-/-;p107-/-, or pRb-/-;p130-/-) or triplicate (wild type, or pRb-/-), in mouse microarrays. Comparison was performed between the 4 different genotypes.
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| Contributor(s) |
Lara MF, García-Escudero R, Ruiz S, Santos M, Moral M, Martínez-Cruz AB, Segrelles C, Lorz C, Paramio JM |
| Citation(s) |
17932948 |
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| Submission date |
Nov 08, 2007 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Ramon Garcia-Escudero |
| E-mail(s) |
ramon.garcia@ciemat.es, rgescudero@gmail.com
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| Organization name |
CIEMAT
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| Department |
Biomedical Innovation
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| Lab |
Molecular and Traslational Oncology
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| Street address |
Avda Complutense 40, Ed 70A
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA103379 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9562_RAW.tar |
32.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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