|
| Status |
Public on Nov 13, 2007 |
| Title |
pRb-/-.3 |
| Sample type |
RNA |
| |
|
| Source name |
pooled primary keratinocyte cultures
|
| Organism |
Mus musculus |
| Characteristics |
age: newborn
strain: FVB
tissue: skin
|
| Growth protocol |
Primary keratinocytes were obtained from newborn mice as described in Santos et al J Biol Chem 277, 19122 (2002). Pooled cells from five different mice of same genotype were cultured in low Ca2+ medium for no longer that 3 days without passage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol and purified using RNeasy Mini kit (Qiagen) following manufacturers' recommendations.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
RNA integrity tested with Bioanalyzer (Agilent)
|
| Data processing |
RMA normalization, log2 transformed, probe value mean-centered, mean=0, stdv=1
|
| |
|
| Submission date |
Nov 08, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Ramon Garcia-Escudero |
| E-mail(s) |
ramon.garcia@ciemat.es, rgescudero@gmail.com
|
| Organization name |
CIEMAT
|
| Department |
Biomedical Innovation
|
| Lab |
Molecular and Traslational Oncology
|
| Street address |
Avda Complutense 40, Ed 70A
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE9562 |
Gene profiling approaches help to define the specific functions of retinoblastoma family in epidermis |
|
