|
| Status |
Public on Sep 15, 2014 |
| Title |
Rv exoquick |
| Sample type |
RNA |
| |
|
| Source name |
Exosomal RNA from M.tb infected macrophages and exosomes purified by exoquick
|
| Organism |
Mycobacterium tuberculosis H37Rv |
| Characteristics |
sample type: Exosomes
infection: M.tb infected
treatment: purification by exoquick
|
| Treatment protocol |
Macrophages were infected with opsonized bacteria so as to achieve 80% infectivity of cells as determined by an uptake assay
|
| Growth protocol |
Murine RAW264.7 macrophages were cultured in complete DMEM media at 37 degrees in a CO2 incubator; Mycobacteria was cultured in 7H9 broth with OADC at 37 degrees incubator shaker
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using MirVana kit (Ambion) and treated with DNAse1 (Invitrogen)
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed using standard labeling procedures from Nimblegen and following manufacture's instructions
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Nimblegen protocols
|
| Scan protocol |
Scanning was performed according to Nimblegen protocol
|
| Description |
Total RNA from exosomes released from M.tb infected RAW264.7 macrophages following exosome purification by exoquick
|
| Data processing |
The raw data was subject to RMA using Nimblescan, quantile normalization
|
| |
|
| Submission date |
Jan 27, 2014 |
| Last update date |
Sep 15, 2014 |
| Contact name |
prachi singh |
| Organization name |
university of notredame
|
| Department |
biological sciences
|
| Street address |
130 Galvin
|
| City |
Notredame |
| State/province |
indiana |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
|
| Platform ID |
GPL18228 |
| Series (1) |
| GSE54444 |
Expression analysis for detection of mycobacterial transcripts present in exosomes derived from M.tb infected macrophages |
|
