|
| Status |
Public on Sep 15, 2014 |
| Title |
Expression analysis for detection of mycobacterial transcripts present in exosomes derived from M.tb infected macrophages |
| Platform organism |
Mycobacterium tuberculosis |
| Sample organism |
Mycobacterium tuberculosis H37Rv |
| Experiment type |
Expression profiling by array
|
| Summary |
Mycobacterial transcripts were identified in exosomes released from M.tb infected RAW264.7 macrophages that were not present in uninfected exosomes suggesting export of mycobacterial RNA via exosomes
Mycobacterial RNA was used as positive control and RNA from exosomes released from uninfected macrophages was used as negative control
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| |
|
| Overall design |
Total RNA isolated from exosomes or mycobacteria was reverse transcribed to ds cDNA which wa slabeled with Cy3 and hybridized to custom whole M.tb genome array from Nimblegen 120910_Mtub_AD_expr.ndf
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| |
|
| Contributor(s) |
Singh P, Schorey J |
| Citation(s) |
25753779 |
| |
| Submission date |
Jan 27, 2014 |
| Last update date |
Mar 16, 2015 |
| Contact name |
prachi singh |
| Organization name |
university of notredame
|
| Department |
biological sciences
|
| Street address |
130 Galvin
|
| City |
Notredame |
| State/province |
indiana |
| ZIP/Postal code |
46556 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18228 |
Custom Mycobacterium Expression Design OID39789 |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA236531 |
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