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Sample GSM1315567 Query DataSets for GSM1315567
Status Public on Sep 15, 2014
Title Un sucrose
Sample type RNA
 
Source name Exosomal RNA from uninfected macrophages and exosomes purified by sucrose gradient
Organism Mycobacterium tuberculosis H37Rv
Characteristics sample type: Exosomes
infection: none
treatment: purification by sucrose gradient
Treatment protocol Macrophages were infected with opsonized bacteria so as to achieve 80% infectivity of cells as determined by an uptake assay
Growth protocol Murine RAW264.7 macrophages were cultured in complete DMEM media at 37 degrees in a CO2 incubator; Mycobacteria was cultured in 7H9 broth with OADC at 37 degrees incubator shaker
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using MirVana kit (Ambion) and treated with DNAse1 (Invitrogen)
Label Cy3
Label protocol Labeling was performed using standard labeling procedures from Nimblegen and following manufacture's instructions
 
Hybridization protocol Hybridization was performed according to Nimblegen protocols
Scan protocol Scanning was performed according to Nimblegen protocol
Description Total RNA from exosomes released from uninfected RAW264.7 macrophages following exosome purification by sucrose gradient
Data processing The raw data was subject to RMA using Nimblescan, quantile normalization
 
Submission date Jan 27, 2014
Last update date Sep 15, 2014
Contact name prachi singh
Organization name university of notredame
Department biological sciences
Street address 130 Galvin
City Notredame
State/province indiana
ZIP/Postal code 46556
Country USA
 
Platform ID GPL18228
Series (1)
GSE54444 Expression analysis for detection of mycobacterial transcripts present in exosomes derived from M.tb infected macrophages

Data table header descriptions
ID_REF
VALUE Average normalized gene level signal intensities

Data table
ID_REF VALUE
GI_15607143:1-1524 864.3533
GI_15607144:1-1209 987.4461
GI_15607145:1-1158 669.477
GI_15607146:1-564 653.0137
GI_15607147:1-2145 672.7834
GI_15607148:1-2517 909.7948
GI_15607149:1-915 1893.822
GI_15607150:1-438 2546.2138
GI_15607151:1-549 4544.5945
GI_15607152:1-426 715.9391
GI_15607153:1-282 432.5192
GI_15607154:1-789 957.3572
GI_15607156:1-1881 1352.736
GI_15607157:1-1296 1170.7885
GI_15607158:1-1476 1186.0099
GI_15607159:1-1410 1121.6785
GI_15607160:1-1545 1146.8597
GI_15607161:1-468 694.3796
GI_15607162:1-1584 1018.9597
GI_15607163:1-969 1495.6513

Total number of rows: 3998

Table truncated, full table size 109 Kbytes.




Supplementary file Size Download File type/resource
GSM1315567_un_sucrose1.pair.gz 1.9 Mb (ftp)(http) PAIR
Processed data included within Sample table

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