|
Status |
Public on Sep 15, 2014 |
Title |
Un sucrose |
Sample type |
RNA |
|
|
Source name |
Exosomal RNA from uninfected macrophages and exosomes purified by sucrose gradient
|
Organism |
Mycobacterium tuberculosis H37Rv |
Characteristics |
sample type: Exosomes infection: none treatment: purification by sucrose gradient
|
Treatment protocol |
Macrophages were infected with opsonized bacteria so as to achieve 80% infectivity of cells as determined by an uptake assay
|
Growth protocol |
Murine RAW264.7 macrophages were cultured in complete DMEM media at 37 degrees in a CO2 incubator; Mycobacteria was cultured in 7H9 broth with OADC at 37 degrees incubator shaker
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using MirVana kit (Ambion) and treated with DNAse1 (Invitrogen)
|
Label |
Cy3
|
Label protocol |
Labeling was performed using standard labeling procedures from Nimblegen and following manufacture's instructions
|
|
|
Hybridization protocol |
Hybridization was performed according to Nimblegen protocols
|
Scan protocol |
Scanning was performed according to Nimblegen protocol
|
Description |
Total RNA from exosomes released from uninfected RAW264.7 macrophages following exosome purification by sucrose gradient
|
Data processing |
The raw data was subject to RMA using Nimblescan, quantile normalization
|
|
|
Submission date |
Jan 27, 2014 |
Last update date |
Sep 15, 2014 |
Contact name |
prachi singh |
Organization name |
university of notredame
|
Department |
biological sciences
|
Street address |
130 Galvin
|
City |
Notredame |
State/province |
indiana |
ZIP/Postal code |
46556 |
Country |
USA |
|
|
Platform ID |
GPL18228 |
Series (1) |
GSE54444 |
Expression analysis for detection of mycobacterial transcripts present in exosomes derived from M.tb infected macrophages |
|