|
| Status |
Public on Jan 01, 2015 |
| Title |
patient 15 |
| Sample type |
genomic |
| |
|
| Source name |
bone marrow from acute lymphoblastic leukemia patient
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: T-ALL
status: newly diagnosed
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from mononuclear cell obtained from bone marrow of ALL patients and healthy donor using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer and agarose gel electrophoresis.
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
Array data were analyzed using Affymetrix Genotyping Console and Chromosome Analysis Suit.
|
| |
|
| Submission date |
Apr 23, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Tian Yuan |
| E-mail(s) |
mdyuantian@163.com
|
| Organization name |
Peking Union Medical College
|
| Department |
Institute of Hematology and Blood Disease Hospital
|
| Lab |
State Key Laboratory of Experimental Hematology
|
| Street address |
No.288 Nanjing Road
|
| City |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE57018 |
Affymetrix SNP array data for adult acute lymphoblastic leukemia samples |
|
