|
| Status |
Public on Oct 10, 2017 |
| Title |
ko12 |
| Sample type |
RNA |
| |
|
| Source name |
kidney of mouse aged about 6 months
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Kidney
grhl1 gene status: Grh1 KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Ron’s FastTRI Extraction Reagent was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Mouse RNA samples (500ng) were processed and labeled for array hybridization using the Ambion WT Expression kit.
|
| |
|
| Hybridization protocol |
Labeled, fragmented cDNA (Affymetrix GeneChip® WT Terminal Labeling and Controls Kit, ) was hybridized to Mouse Gene 2.0 arrays for 16 hours at 45°C (at 60 rpm) (Affymetrix GeneChip® Hybridization, Wash, and Stain Kit). Arrays were washed and stained using the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner 3000 7G.
|
| Description |
Gene expression data from kidney of Grhl1wt and Grhl1 ko mice
|
| Data processing |
The data were analyzed using R/Bioconductor statistical environment. Intensities of microarrays were log2-transformed and normalized by RMA method.
|
| |
|
| Submission date |
Oct 10, 2014 |
| Last update date |
Oct 10, 2017 |
| Contact name |
Tomasz Wilanowski |
| Organization name |
Nencki Institute of Experimental Biology, Polish Academy of Sciences
|
| Department |
Department of Cell Biology
|
| Lab |
Laboratory of Signal Transduction
|
| Street address |
3 Pasteur Street
|
| City |
Warsaw |
| State/province |
Mazowieckie |
| ZIP/Postal code |
02-093 |
| Country |
Poland |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE62252 |
Expression data from kidney of Grhl1wt and Grhl1 ko mice. |
|
