|
| Status |
Public on Mar 31, 2017 |
| Title |
Aug_23_2013_at_10:00_in_Fukuoka_DPS_78 |
| Sample type |
RNA |
| |
|
| Source name |
rice, leaf
|
| Organism |
Oryza sativa |
| Characteristics |
variety: Koshihikari
place: Fukuoka
sampling date: 8-23-13 10:00
days from transplanting to sampling: 78
tissue: leaf
|
| Treatment protocol |
No particular treatment. The plants were grown according to practical cultivation at the sites.
|
| Growth protocol |
The rice seedlings were transplanted on 2013/6/5, or 2013/6/27. The rice plants were grown in Fukuoka field.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Promega Maxwell 16 Instrument following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
200 ng of total RNA were reverse-transcribed and labelled using Low Input Quick Amp Labeling Kit following manufacturer's instructions. Labelled cRNA were purified with Qiagen RNeasy mini kit.
|
| |
|
| Hybridization protocol |
825ng of labelled cRNA were hybridized using Gene Expression Hybridization Kit following manufacturer's instructions at NIAS RGRC Open Laboratory.
|
| Scan protocol |
Scanned with Agilent G2505C.
|
| Description |
03_13_0065
raw data file: S254663310117_A4.txt
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.5.1.1).
|
| |
|
| Submission date |
Feb 04, 2015 |
| Last update date |
Mar 31, 2017 |
| Contact name |
Takeshi Izawa |
| Organization name |
National Institute of Agrobiological Sciences
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL19684 |
| Series (1) |
| GSE65599 |
Diurnal variation for rice leaves in Fukuoka field in 2013 |
|
