|
| Status |
Public on Mar 31, 2016 |
| Title |
NSK_HOD2_20Tags |
| Sample type |
SRA |
| |
|
| Source name |
Random DNA sequences
|
| Organism |
synthetic construct |
| Characteristics |
molecule type: DNA barcodes
host cell line: PC3
|
| Treatment protocol |
Lipofectamine-RNAiMAX transfection with DNA barcodes
|
| Growth protocol |
PC3 cells maintained in RPMI-Glutamax medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA extraction from Qiagen
P5 and P7 adapters by blunt end ligation to DNA barcodes eluted from E-gel size select post 10cycle PCR at 60°C
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
20 random DNA barcodes flanked by pGEX adapters
|
| Data processing |
FASTQC initial quality and copy number analysis. This is the only result needed for the validating SRiD: DNA barcode generator and management system. Further alignments are not necessary for our project.
Copy numbers are generated automatically by FASTQC software during the initial quality check-up step.
Here, the term 'copy number' refers to the final number of individual DNA barcodes/sequences that are significantly represented in a high throughput sequencing run.
Supplementary_files_format_and_content: FASTQC output which includes number of significant sequences and their respective percent representation in our screen.
|
| |
|
| Submission date |
Mar 31, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
sandeep kumar Nadendla |
| E-mail(s) |
nsknadendla@gmail.com
|
| Phone |
0033438780293
|
| Organization name |
CEA
|
| Department |
iRTSV/Biomics
|
| Lab |
Biomics
|
| Street address |
17 Rue des martyrs
|
| City |
Grenoble |
| State/province |
Isere |
| ZIP/Postal code |
38000 |
| Country |
France |
| |
|
| Platform ID |
GPL17769 |
| Series (1) |
| GSE67441 |
SRiD- A facile DNA barcode generation and management system for high throughput screening |
|
| Relations |
| BioSample |
SAMN03452278 |
| SRA |
SRX973921 |
