|
| Status |
Public on Aug 01, 2015 |
| Title |
N1-D/RBP-AM-Ert2+Dox_1_vs_DPNII |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
N1-D/RBP-AM-Ert2+Dox_1
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
cell line: mK4
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Dneasy extraction kit, Dpn1 digestion, adaptor ligation, and adaptor mediated PCR for library generation
|
| Label |
Cy5
|
| Label protocol |
Nimblegen labeling protocol performed by MoGene Inc.
|
| |
|
| Channel 2 |
| Source name |
DpnII background
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
cell line: mK4
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Dneasy extraction kit, Dpn1 digestion, adaptor ligation, and adaptor mediated PCR for library generation
|
| Label |
Cy3
|
| Label protocol |
Nimblegen labeling protocol performed by MoGene Inc.
|
| |
|
| |
| Hybridization protocol |
Nimblegen hybridization protocol performed by MoGene Inc
|
| Scan protocol |
performed by MoGene Inc
|
| Description |
promoter array analysis
|
| Data processing |
Signal was mapped to DpnI fragments and compared against signal produced by D/AM controls
Experimental signal was compared against D/AM control signal and scored based on anova analysis of replicates to identify DpnI fragments showing enrichment over control
|
| |
|
| Submission date |
Jun 29, 2015 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Raphael Kopan |
| E-mail(s) |
rafi.kopan@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Developmental Biology
|
| Lab |
Kopan
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL17878 |
| Series (2) |
| GSE70388 |
SpDamID: Marking DNA Bound by Protein Complexes Identifies Notch-Dimer Responsive Enhancers [array] |
| GSE70402 |
SpDamID: Marking DNA Bound by Protein Complexes Identifies Notch-Dimer Responsive Enhancers |
|
