|
| Status |
Public on Aug 06, 2016 |
| Title |
pstS::Tn5;ΔphoU;Pvan-phoU -vanillate/+vanillate 16 hours depletion rep 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mixed population in PYE + vanillate
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype: pstS::Tn5;ΔphoU;Pvan-phoU
treatment: 50 mM vanillate
|
| Growth protocol |
Cells were grown to mid-log phase in rich medium (PYE) supplemented with 50 mM vanillate, washed, and released into PYE with or without vanillate. Cells were grown in mid-log phase for the specified number of hours before sampling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNAeasy kit (Qiagen) according to manufacturer's protoco
|
| Label |
Cy3
|
| Label protocol |
RNA was reverse transcribed into cDNA as described previously (Laub et al. 2000)
|
| |
|
| Channel 2 |
| Source name |
mixed population in PYE - vanillate
|
| Organism |
Caulobacter vibrioides NA1000 |
| Characteristics |
genotype: pstS::Tn5;ΔphoU;Pvan-phoU
treatment: without vanillate
|
| Growth protocol |
Cells were grown to mid-log phase in rich medium (PYE) supplemented with 50 mM vanillate, washed, and released into PYE with or without vanillate. Cells were grown in mid-log phase for the specified number of hours before sampling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNAeasy kit (Qiagen) according to manufacturer's protoco
|
| Label |
Cy5
|
| Label protocol |
RNA was reverse transcribed into cDNA as described previously (Laub et al. 2000)
|
| |
|
| |
| Hybridization protocol |
Agilent protocol
|
| Scan protocol |
Arrays were scanned using Agilent Technologies Scanner G2505C
Arrays were scanned using Agilent Technologies Scanner G2505C
|
| Description |
Cells grown in PYE + vanillate to mid-log phase, washed, and released into PYE + or - vanillate and grown in mid-log phase for the specified number of hours
|
| Data processing |
Data were retrieved using Agilent Feature Extractor 10.6
|
| |
|
| Submission date |
Aug 07, 2015 |
| Last update date |
Aug 06, 2016 |
| Contact name |
Michael Laub |
| Organization name |
MIT
|
| Street address |
31 Ames St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL15487 |
| Series (2) |
| GSE71857 |
Identification of the PhoB regulon and role of PhoU in the phosphate-starvation response of Caulobacter crescentus (expression) |
| GSE71961 |
Identification of the PhoB regulon and role of PhoU in the phosphate-starvation response of Caulobacter crescentus |
|
