|
| Status |
Public on Jun 10, 2016 |
| Title |
liver-m-VPS33B-ko-1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse liver, male, Vps33b liver-sepcific ko
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
genotype/variation: Vps33b liver-sepcific ko
Sex: male
|
| Growth protocol |
10 week old mice were fed 0.5% cholic acid supplemented chow (TestDiet® Europe, London, UK) for 2 weeks and sacrificed immediately after
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed using the RNAeasy Microkit (Qiagen, UK)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix Mogene 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
RMA processing using 'oligo' Bioconductor package in R
|
| |
|
| Submission date |
Jun 09, 2016 |
| Last update date |
Jun 10, 2016 |
| Contact name |
Paul Gissen |
| Organization name |
UCL
|
| Department |
LMCB
|
| Street address |
Gower Street
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE83192 |
Liver tissue from liver specific Vps33b knockout (Vps33bfl/fl-AlfpCre) and control (Vps33bfl/fl) mice |
|
