|
| Status |
Public on Jul 15, 2016 |
| Title |
implantation sites on day 12 in IVT group |
| Sample type |
RNA |
| |
|
| Source name |
uterus tissue at implantation sites on day 12 (day 0 = day of estrous beginning) in IVT group
|
| Organism |
Ovis aries |
| Characteristics |
breed: Ujumqin
tissue: uterus tissue
embryo: in vitro embryo
day: 12
|
| Treatment protocol |
All uterus samples were frozen in liquid nitrogen for RNA extraction.
|
| Growth protocol |
21 Ujumqin ewes aged 10 months that were used in this study were housed at Beijing Waddell sheep breeding farm (Beijing, China) under similar environmental and nutritional conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
10 ng RNA samples was reverse transcripted and labeled by biotin with GeneChip Pico WT Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
20µg fragmented cRNA was prepared, hybridized to each GeneChip strip, washed, stained with streptavidin phycoerythrinonan (SAPE).
|
| Scan protocol |
GeneChips were scanned according to the manufacturer's protocols (Affymetrix GeneAtlas® Instrument).
|
| Description |
Gene expression data from uterus tissue at implantation sites on day 12 (day 0 = day of estrous beginning) in IVT group
|
| Data processing |
Quantile normalization of microarray data and calculation of gene expression values were conducted by the bioconductor package (http://www.bioconductor.org) using the Robust Multiarray Average (RMA) method.
|
| |
|
| Submission date |
Jul 14, 2016 |
| Last update date |
Jul 15, 2016 |
| Contact name |
Xihui Sheng |
| E-mail(s) |
shengxh03@163.com
|
| Organization name |
Beijing university of agriculture
|
| Street address |
No.7 Beinong Road, Huilongguan Town, Changping District
|
| City |
Beijing |
| ZIP/Postal code |
102206 |
| Country |
China |
| |
|
| Platform ID |
GPL19480 |
| Series (1) |
| GSE84417 |
Expression data from uterus tissue of sheep |
|
