|
| Status |
Public on Nov 29, 2016 |
| Title |
WT_MP_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Nigrostriatal tissue isolated from brain of mice treated with MP, replicate 2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brain (nigrostriatal tissue)
gender: male
genotype: wild type
strain: C57BL6/J
age: adult
|
| Treatment protocol |
Adult (8-10-week- old), pathogen-free male mice (C57BL6/J from Jackson Laboratories, Bar Harbor, Maine) were housed one to a cage for a week for acclimatization before experimental dosing. Mice that contained disrupted α-synuclein (α-synuclein knockout mice, stock number 003692) were treated similarly. Age-matched wild type C57BL6/J mice that contained α-synuclein served as the control animals for knockout experiments. The animals were housed under standard conditions of temperature and humidity with a 12 h light/dark cycle and fed a standard pellet diet and water ad libitum. Animals were treated with normal saline (control), paraquat (10mg/kg, ip) and Maneb (30mg/kg, ip) twice a week for 6 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the nigrostriatal tissue of three independent triplicates using the TRIzol (Ambion, Life Technologies, Grand Island, NY) and RNeasy mini kit columns with in-column DNAse treatment (Qiagen, Valencia, CA).
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 3 ug of Cy3 labeled cDNA was hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Gene expression of WT mice after treatment with MP
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v12), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Nov 04, 2016 |
| Last update date |
Nov 30, 2016 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE89562 |
Role of cytochrome c in α-synuclein radical formation: implications of α-synuclein in neuronal death in Maneb and paraquat-induced model of Parkinson's disease |
|
