|
| Status |
Public on Jul 24, 2017 |
| Title |
Line 49-G24 - mother |
| Sample type |
SRA |
| |
|
| Source name |
leaf tissue
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
tissue: leaf
age: ~3 weeks old
|
| Growth protocol |
All samples in this study are the Col-0 ecotype of Arabidopsis thaliana. Plants were grown in long-day (16 hour light) conditions at 24℃ and 3 week old leaf tissue was used for all experiments and sequencing.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was isolated using fractional precipitation followed by phenol-chloroform extraction and RNase A treatment. 1ug of DNA was used to prepare libraries as previously described (Urich et al. 2015).
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
MA-cross_line49-G24
|
| Data processing |
Library strategy: MethylC–seq
Data were mapped to the TAIR10 reference genome
MethylC-seq analysis were performed as described in Schultz, M. D. et al. Human body epigenome maps reveal noncanonical DNA methylation variation. Nature 523, 212-216 (2015).
Genome_build: TAIR10
Supplementary_files_format_and_content: tab delimited text file; <chromosome> <cytosine position> <strand> <CNN (N = A, T, C, G)> <methylated cytosines at this site> <mapped cytosines at this site> <binomial test, 1 means methylated, 0 means unmethylated>
|
| |
|
| Submission date |
Jan 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
schmitz@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platform ID |
GPL19580 |
| Series (1) |
| GSE94298 |
Stable Inheritance of Spontaneous Epialleles in Arabidopsis thaliana |
|
| Relations |
| BioSample |
SAMN06282862 |
| SRA |
SRX2528548 |
